ELISA/assay

Mouse Heparin Cofactor II ELISA Kit

MBS731379

96 Strip Wells

675 USD

ELISA Kit

Mouse Heparin Cofactor II ELISA Kit

Heparin Cofactor II

heparin cofactor 2; Heparin cofactor 2; heparin cofactor 2; serpin D1; leuserpin 2; protease inhibitor leuserpin-2; serine (or cysteine) proteinase inhibitor, clade D (heparin cofactor), member 1; serpin peptidase inhibitor, clade D (heparin cofactor), member 1; Heparin cofactor II; HC-II; Protease inhibitor leuserpin-2; HLS2; Serpin D1

HC II

SERPIND1; SERPIND1; HC2; LS2; HCF2; HCII; HLS2; THPH10; D22S673; HCF2; HC-II; HLS2

HEP2_HUMAN

Mouse

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Intended Uses: This HCII ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of mouse HCII. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Sensitivity: The sensitivity in this assay is 0.1 ng/mL.

Cardiovascular

Principle of the Assay: HCII ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HCII antibody and an HCII-HRP conjugate. The assay sample and buffer are incubated together with HCII-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HCII concentration since HCII from samples and HCII-HRP conjugate compete for the anti-HCII antibody binding site. Since the number of sites is limited, as more sites are occupied by HCII from the sample, fewer sites are left to bind HCII-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HCII concentration in each sample is interpolated from this standard curve.

73858566

NP_000176.2

NM_000185.3

57,071 Da

Complement And Coagulation Cascades Pathway 198880!!Complement And Coagulation Cascades Pathway 83073!!Complement And Coagulation Cascades Pathway 484

The product encoded by this gene is a serine proteinase inhibitor which rapidly inhibits thrombin in the presence of dermatan sulfate or heparin. The gene contains five exons and four introns. This protein shares homology with antithrombin III and other members of the alpha 1-antitrypsin superfamily. Mutations in this gene are associated with heparin cofactor II deficiency. [provided by RefSeq, Jul 2008]

96 Strip Wells

675 USD