ELISA/assay

Human Anti thymocyte globulin ELISA Kit

MBS731343

96 Strip Wells

675 USD

ELISA Kit

Human Anti thymocyte globulin ELISA Kit

Anti thymocyte globulin

ATG

Human

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Sensitivity: The sensitivity in this assay is 1.0 pg/mL.

Immunology

Intended Uses: This ATG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ATG. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: ATG ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ATG antibody and an ATG-HRP conjugate. The assay sample and buffer are incubated together with ATG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATG concentration since ATG from samples and ATG-HRP conjugate compete for the anti-ATG antibody binding site. Since the number of sites is limited, as more sites are occupied by ATG from the sample, fewer sites are left to bind ATG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATG concentration in each sample is interpolated from this standard curve.

96 Strip Wells

675 USD