catalog number :
MBS731336
products type :
ELISA Kit
products full name :
Human beta Defensin 3 ELISA Kit
products short name :
beta Defensin 3
products name syn :
Human b Defensin 3 ELISA Kit
other names :
Defensin-B3; Defensin-B3
products gene name :
DEFb3
products gene name syn :
DEFb3
other gene names :
DefB3; OaDefB3
uniprot entry name :
DEFB3_ORNAN
specificity :
This assay has high sensitivity and excellent specificity for detection of HbetaD-3. No significant cross-reactivity or interference between HbetaD-3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HbetaD-3 and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.
products categories :
Immunology
products description :
Intended Uses: This HbetaD-3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human HbetaD-3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay HbetaD-3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HbetaD-3 antibody and an HbetaD-3-HRP conjugate. The assay sample and buffer are incubated together with HbetaD-3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HbetaD-3 concentration since HbetaD-3 from samples and HbetaD-3-HRP conjugate compete for the anti-HbetaD-3 antibody binding site. Since the number of sites is limited, as more sites are occupied by HbetaD-3 from the sample, fewer sites are left to bind HbetaD-3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HbetaD-3 concentration in each sample is interpolated from this standard curve.
ncbi mol weight :
7,408 Da