ELISA/assay

Human Protein Tyrosine Phosphatase Receptor Type N ELISA Kit

MBS731293

48-Strip-Wells

470 USD

ELISA Kit

Human Protein Tyrosine Phosphatase Receptor Type N ELISA Kit

Protein Tyrosine Phosphatase Receptor Type N

PTPRN protein, partial; Receptor-type tyrosine-protein phosphatase-like N; receptor-type tyrosine-protein phosphatase-like N; ICA 512; PTP IA-2; islet cell antigen 2; islet cell antigen 512; islet cell autoantigen 3; protein tyrosine phosphatase-like N; protein tyrosine phosphatase, receptor type, N; Islet cell antigen 512; ICA 512; Islet cell autoantigen 3; PTP IA-2

PTPRN

PTPRN; PTPRN; IA2; IA-2; ICA512; R-PTP-N; IA-2/PTP; ICA3; ICA512; R-PTP-N; ICA 512

PTPRN_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of PTPRN. No significant cross-reactivity or interference between PTPRN and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PTPRN and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Signal Transduction

Intended Uses: This PTPRN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PTPRN. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay PTPRN ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PTPRN antibody and an PTPRN-HRP conjugate. The assay sample and buffer are incubated together with PTPRN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PTPRN concentration since PTPRN from samples and PTPRN-HRP conjugate compete for the anti-PTPRN antibody binding site. Since the number of sites is limited, as more sites are occupied by PTPRN from the sample, fewer sites are left to bind PTPRN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PTPRN concentration in each sample is interpolated from this standard curve.

39644800

AAH07713.2

96,261 Da

Type I Diabetes Mellitus Pathway (83095); Type I Diabetes Mellitus Pathway (507)

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and a single catalytic domain, and thus represents a receptor-type PTP. This PTP was found to be an autoantigen that is reactive with insulin-dependent diabetes mellitus (IDDM) patient sera, and thus may be a potential target of autoimmunity in diabetes mellitus. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Dec 2010]

PTPRN: Implicated in neuroendocrine secretory processes. May be involved in processes specific for neurosecretory granules, such as their biogenesis, trafficking or regulated exocytosis or may have a general role in neuroendocrine functions. Seems to lack intrinsic enzyme activity. May play a role in the regulation of secretory granules via its interaction with SNTB2. Belongs to the protein-tyrosine phosphatase family. Receptor class 8 subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Receptor protein phosphatase, tyrosine. Chromosomal Location of Human Ortholog: 2q35-q36.1. Cellular Component: integral to membrane. Molecular Function: protein binding; GTPase binding; spectrin binding; protein tyrosine phosphatase activity. Biological Process: response to reactive oxygen species; response to cAMP; response to estrogen stimulus; insulin secretion; cytokine and chemokine mediated signaling pathway; response to glucose stimulus; protein amino acid dephosphorylation; response to insulin stimulus

48-Strip-Wells

470 USD

96-Strip-Wells

675