ELISA/assay

Rat Matrix Metalloproteinase 7 ELISA Kit

MBS731282

48-Strip-Wells-(Comp

470 USD

ELISA Kit

Rat Matrix Metalloproteinase 7 ELISA Kit

Matrix Metalloproteinase 7

MMP-7

Rat

Store all reagents at 2-8 degree C.

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 0.1 ng/mL.

Cancer

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate . INTENDED USE This MMP-7 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat MMP-7. This ELISA kit for research use only, not for therapeutic or diagnostic applications! . PRINCIPLE OF THE ASSAY MMP-7 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MMP-7 antibody and an MMP-7-HRP conjugate. The assay sample and buffer are incubated together with MMP-7-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MMP-7 concentration since MMP-7 from samples and MMP-7-HRP conjugate compete for the anti-MMP-7 antibody binding site. Since the number of sites is limited, as more sites are occupied by MMP-7 from the sample, fewer sites are left to bind MMP-7-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MMP-7 concentration in each sample is interpolated from this standard curve.

48-Strip-Wells-(Competitive)

470 USD

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675