Rat CD 40 Ligand ELISA Kit | MyBioSource MBS731275 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat CD 40 Ligand ELISA Kit

catalog :

MBS731275

quantity :

48-Strip-Wells-(Comp

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS731275

products type :

ELISA Kit

products full name :

Rat CD 40 Ligand ELISA Kit

products short name :

CD 40 Ligand

other names :

CD40 ligand; CD40 ligand; CD40 ligand; CD40-L; CD40 antigen ligand; T-cell antigen Gp39; T-B cell-activating molecule; TNF-related activation protein; tumor necrosis factor (ligand) superfamily member 5; CD40 ligand; T-cell antigen Gp39; TNF-related activation protein; TRAP; Tumor necrosis factor ligand superfamily member 5; CD_antigen: CD154Cleaved into the following 2 chains:CD40 ligand, membrane form; CD40 ligand, soluble form

products gene name :

CD40L

other gene names :

CD40LG; CD40LG; IGM; IMD3; TRAP; gp39; CD154; CD40L; HIGM1; T-BAM; TNFSF5; hCD40L; CD40L; TNFSF5; TRAP; CD40-L; TRAP

uniprot entry name :

CD40L_HUMAN

reactivity :

Rat

specificity :

This assay has high sensitivity and excellent specificity for detection of CD40L. No significant cross-reactivity or interference between CD40L and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CD40L and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 0.1 ng/mL.

other info2 :

Intended Uses: This CD40L ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat CD40L. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Immunology

products description :

Principle of the assay: CD40L ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CD40L antibody and an CD40L-HRP conjugate. The assay sample and buffer are incubated together with CD40L-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CD40L concentration since CD40L from samples and CD40L-HRP conjugate compete for the anti-CD40L antibody binding site. Since the number of sites is limited, as more sites are occupied by CD40L from the sample, fewer sites are left to bind CD40L-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD40L concentration in each sample is interpolated from this standard curve.

ncbi gi num :

4557433

ncbi acc num :

NP_000065.1

ncbi gb acc num :

NM_000074.2

ncbi mol weight :

29,274 Da

ncbi pathways :

Adaptive Immune System Pathway (366160); Allograft Rejection Pathway (920963); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Asthma Pathway (83120); Asthma Pathway (532); Autoimmune Thyroid Disease Pathway (83121); Autoimmune Thyroid Disease Pathway (533); CD40/CD40L Signaling Pathway (138061); Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway (137993)

ncbi summary :

The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq, Jul 2008]

uniprot summary :

CD40LG: Mediates B-cell proliferation in the absence of co- stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching. Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1); also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence. Belongs to the tumor necrosis factor family. Protein type: Membrane protein, integral; Cell adhesion. Chromosomal Location of Human Ortholog: Xq26. Cellular Component: extracellular space; integral to plasma membrane; integral to membrane; plasma membrane; external side of plasma membrane. Molecular Function: cytokine activity; CD40 receptor binding; tumor necrosis factor receptor binding. Biological Process: B cell proliferation; platelet activation; leukocyte adhesion; regulation of immune response; positive regulation of interleukin-12 production; B cell differentiation; immunoglobulin secretion; isotype switching; inflammatory response; regulation of immunoglobulin secretion; negative regulation of apoptosis. Disease: Immunodeficiency With Hyper-igm, Type 1

size1 :

48-Strip-Wells-(Competitive)

price1 :

470 USD

size2 :

48-Strip-Wells-(Sandwich)

price2 :

470

size3 :

96-Strip-Wells-(Competitive)

price3 :

675

size4 :

96-Strip-Wells-(Sandwich)

price4 :

675

more info or order :

MyBioSource product webpage