ELISA/assay

Rabbit Prothrombin Fragment 1+2 ELISA Kit

MBS731253

48-Strip-Wells-(Comp

470 USD

ELISA Kit

Rabbit Prothrombin Fragment 1+2 ELISA Kit

Prothrombin Fragment 1+2

PF1+2

Rabbit

Store all reagents at 2-8 degree C.

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 0.1 ng/mL.

Cardiovascular

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate . INTENDED USE This PF1+2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit PF1+2. This ELISA kit for research use only, not for therapeutic or diagnostic applications! . PRINCIPLE OF THE ASSAY PF1+2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PF1+2 antibody and an PF1+2-HRP conjugate. The assay sample and buffer are incubated together with PF1+2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PF1+2 concentration since PF1+2 from samples and PF1+2-HRP conjugate compete for the anti-PF1+2 antibody binding site. Since the number of sites is limited, as more sites are occupied by PF1+2 from the sample, fewer sites are left to bind PF1+2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PF1+2 concentration in each sample is interpolated from this standard curve.

48-Strip-Wells-(Competitive)

470 USD

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675