ELISA/assay

Rabbit A Disintegrin And Metalloproteinase With Thrombospondin 1 ELISA Kit

MBS731242

48-Strip-Wells

470 USD

ELISA Kit

Rabbit A Disintegrin And Metalloproteinase With Thrombospondin 1 ELISA Kit

A Disintegrin And Metalloproteinase With Thrombospondin 1

A disintegrin and metalloproteinase with thrombospondin motifs 1 preproprotein; A disintegrin and metalloproteinase with thrombospondin motifs 1; A disintegrin and metalloproteinase with thrombospondin motifs 1; METH-1; ADAM-TS1; ADAMTS-1; ADAM-TS 1; human metalloproteinase with thrombospondin type 1 motifs; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 1; ADAM metallopeptidase with thrombospondin type 1 motif, 1; METH-1

ADAMTS1

ADAMTS1; ADAMTS1; C3-C5; METH1; KIAA1346; METH1; ADAM-TS 1; ADAM-TS1; ADAMTS-1

ATS1_HUMAN

Rabbit

This assay has high sensitivity and excellent specificity for detection of ADAMTS1. No significant cross-reactivity or interference between ADAMTS1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ADAMTS1 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Intended Uses: This ADAMTS1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit ADAMTS1. This ELISA kit for research use only, not for therapeutic or diagnostic applications

Cardiovascular

Principle of the assay: ADAMTS1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ADAMTS1 antibody and an ADAMTS1-HRP conjugate. The assay sample and buffer are incubated together with ADAMTS1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADAMTS1 concentration since ADAMTS1 from samples and ADAMTS1-HRP conjugate compete for the anti-ADAMTS1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ADAMTS1 from the sample, fewer sites are left to bind ADAMTS1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADAMTS1 concentration in each sample is interpolated from this standard curve.

50845384

NP_008919.3

NM_006988.3

105,358 Da

Degradation Of The Extracellular Matrix Pathway (576263); Endochondral Ossification Pathway (198812); Extracellular Matrix Organization Pathway (576262); Metabolism Of Proteins Pathway (106230); O-glycosylation Of TSR Domain-containing Proteins Pathway (980460); O-linked Glycosylation Pathway (980459); Post-translational Protein Modification Pathway (161012)

This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motif) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The protein encoded by this gene contains two disintegrin loops and three C-terminal TS motifs and has anti-angiogenic activity. The expression of this gene may be associated with various inflammatory processes as well as development of cancer cachexia. This gene is likely to be necessary for normal growth, fertility, and organ morphology and function. [provided by RefSeq, Jul 2008]

ADAMTS1: Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. Has angiogenic inhibitor activity. Active metalloprotease, which may be associated with various inflammatory processes as well as development of cancer cachexia. May play a critical role in follicular rupture. Protein type: Protease; Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted; EC 3.4.24.-. Chromosomal Location of Human Ortholog: 21q21.2. Cellular Component: cytoplasm; basement membrane; cytoplasmic vesicle. Molecular Function: heparin binding; zinc ion binding; metallopeptidase activity; metalloendopeptidase activity. Biological Process: integrin-mediated signaling pathway; negative regulation of cell proliferation; ovulation from ovarian follicle; proteolysis; kidney development

48-Strip-Wells

470 USD

96-Strip-Wells

675