ELISA/assay

Guinea pig C Reactive Protein ELISA Kit

MBS731198

96 Strip Wells

675 USD

ELISA Kit

Guinea pig C Reactive Protein ELISA Kit

C Reactive Protein

CRP; C-reactive protein; C-reactive protein; pentraxin 1; C-reactive protein, pentraxin-related

CRP

CRP; CRP; PTX1; PTX1

CRP_HUMAN

Guinea Pig

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Intended Uses: This CRP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea Pig CRP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Sensitivity: The sensitivity in this assay is 1.0 ng/mL.

Immunology

Principle of the Assay: CRP ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CRP. Standards or samples are then added to the microtiter plate wells and CRP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CRP present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for CRP are added to each well to "sandwich" the CRP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CRP and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CRP concentration in each sample is interpolated from this standard curve.

17975775

AAL48218.2

10,415 Da

Classical Antibody-mediated Complement Activation Pathway 106409!!Complement Cascade Pathway 106405!!Creation Of C4 And C2 Activators Pathway 106407!!IL6-mediated Signaling Events Pathway 137932!!Immune System Pathway 106386!!Initial Triggering Of Complement Pathway 106406!!Innate Immune System Pathway 106387!!Selenium Pathway 198825

The protein encoded by this gene belongs to the pentaxin family. It is involved in several host defense related functions based on its ability to recognize foreign pathogens and damaged cells of the host and to initiate their elimination by interacting with humoral and cellular effector systems in the blood. Consequently, the level of this protein in plasma increases greatly during acute phase response to tissue injury, infection, or other inflammatory stimuli. [provided by RefSeq, Sep 2009]

96 Strip Wells

675 USD