ELISA/assay

Mouse Insulin receptor substrate 2 ELISA Kit

MBS731161

48-Strip-Wells

470 USD

ELISA Kit

Mouse Insulin receptor substrate 2 ELISA Kit

Insulin receptor substrate 2

insulin receptor substrate 2; Insulin receptor substrate 2; insulin receptor substrate 2; insulin receptor substrate 2

IRS-2

IRS2; IRS2; IRS-2; IRS-2

IRS2_HUMAN

Mouse

This assay has high sensitivity and excellent specificity for detection of IRS2. No significant cross-reactivity or interference between IRS2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IRS2 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This IRS2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse IRS2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the assay: IRS2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IRS2 antibody and an IRS2-HRP conjugate. The assay sample and buffer are incubated together with IRS2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IRS2 concentration since IRS2 from samples and IRS2-HRP conjugate compete for the anti-IRS2 antibody binding site. Since the number of sites is limited, as more sites are occupied by IRS2 from the sample, fewer sites are left to bind IRS2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IRS2 concentration in each sample is interpolated from this standard curve.

38683860

NP_003740.2

NM_003749.2

137,334 Da

AMPK Signaling Pathway (989139); AMPK Signaling Pathway (992181); Adaptive Immune System Pathway (366160); Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); Adipogenesis Pathway (198832); Alpha6-Beta4 Integrin Signaling Pathway (198807); BDNF Signaling Pathway (712093); Constitutive PI3K/AKT Signaling In Cancer Pathway (685535); Cytokine Signaling In Immune System Pathway (366171)

This gene encodes the insulin receptor substrate 2, a cytoplasmic signaling molecule that mediates effects of insulin, insulin-like growth factor 1, and other cytokines by acting as a molecular adaptor between diverse receptor tyrosine kinases and downstream effectors. The product of this gene is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation, as well as by an interleukin 4 receptor-associated kinase in response to IL4 treatment. [provided by RefSeq, Jul 2008]

IRS2: May mediate the control of various cellular processes by insulin. Interacts with PHIP. Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold. Chromosomal Location of Human Ortholog: 13q34. Cellular Component: plasma membrane; cytosol. Molecular Function: protein domain specific binding; protein binding; signal transducer activity; phosphoinositide 3-kinase binding; protein kinase binding; protein phosphatase binding; insulin receptor binding. Biological Process: negative regulation of kinase activity; regulation of lipid metabolic process; nerve growth factor receptor signaling pathway; positive regulation of glycogen biosynthetic process; lipid homeostasis; signal transduction; positive regulation of glucose import; mammary gland development; positive regulation of mesenchymal cell proliferation; positive regulation of cell proliferation; response to glucose stimulus; positive regulation of B cell proliferation; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; glucose metabolic process; positive regulation of insulin secretion; positive regulation of fatty acid beta-oxidation; cell proliferation; cellular response to insulin stimulus; insulin receptor signaling pathway; innate immune response; brain development; negative regulation of B cell apoptosis; positive regulation of cell migration. Disease: Diabetes Mellitus, Noninsulin-dependent

48-Strip-Wells

470 USD

96-Strip-Wells

675