ELISA/assay

Rat Angiotensin II Converting Enzyme ELISA Kit

MBS731127

48-Strip-Wells

440 USD

ELISA Kit

Rat Angiotensin II Converting Enzyme ELISA Kit

[Angiotensin II Converting Enzyme]

[Rat Angiotensin II Converting Enzyme ELISA Kit; Angiotensin II Converting Enzyme; Angiotensin II Converting Enzyme (Rat)]

[angiotensin-converting enzyme 2; Angiotensin-converting enzyme 2; angiotensin-converting enzyme 2; OTTHUMP00000022963; metalloprotease MPROT15; ACE-related carboxypeptidase; angiotensin I converting enzyme 2; angiotensin-converting enzyme homolog; angiotensin I converting enzyme (peptidyl-dipeptidase A) 2; ACE-related carboxypeptidase; Angiotensin-converting enzyme homolog; ACEH; Metalloprotease MPROT15]

[ACE II]

[ACE2; ACE2; ACEH; DKFZp434A014]

ACE2_HUMAN

Rat

This assay has high sensitivity and excellent specificity for detection of ACE2. No significant cross-reactivity or interference between ACE2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ACE2 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Quantitative Competitive. Sensitivity: 1.0 pg/mL

Rat ELISA Kit

Intended Uses: This ACE2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat ACE2. Principle of the Assay: ACE2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ACE2 antibody and an ACE2-HRP conjugate. The assay sample and buffer are incubated together with ACE2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACE2 concentration since ACE2 from samples and ACE2-HRP conjugate compete for the anti-ACE2 antibody binding site. Since the number of sites is limited, as more sites are occupied by ACE2 from the sample, fewer sites are left to bind ACE2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACE2 concentration in each sample is interpolated from this standard curve.

11225609

NP_068576.1

NM_021804.2

Q9BYF1

92,463 Da

ACE Inhibitor Pathway (198763); Protein Digestion And Absorption Pathway (172847); Protein Digestion And Absorption Pathway (171868); Renin-angiotensin System Pathway (83075); Renin-angiotensin System Pathway (486)

The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq]

ACE2: Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin- 13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses. Interacts with ITGB1. Interacts with SARS-CoV and HCoV- NL63 spike glycoprotein. Up-regulated in failing heart. Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells. Expressed in heart, kidney, testis, and gastrointestinal system. Activated by chloride and fluoride, but not bromide. Inhibited by MLN-4760, cFP_Leu, and EDTA, but not by the ACE inhibitors linosipril, captopril and enalaprilat. Belongs to the peptidase M2 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Protease; Membrane protein, integral; EC 3.4.17.23. Chromosomal Location of Human Ortholog: Xp22. Cellular Component: extracellular space; cell surface; cytoplasm; extracellular region; integral to membrane; plasma membrane; lipid raft. Molecular Function: peptidyl-dipeptidase activity; viral receptor activity; protein binding; metallopeptidase activity; zinc ion binding; carboxypeptidase activity; peptide hormone binding; endopeptidase activity; glycoprotein binding. Biological Process: virion attachment, binding of host cell surface receptor; entry of virus into host cell; maternal process involved in pregnancy; regulation of vasodilation; angiotensin maturation; proteolysis; regulation of systemic arterial blood pressure by renin-angiotensin; regulation of cytokine production; regulation of cell proliferation; cellular protein metabolic process; regulation of vasoconstriction; angiotensin catabolic process in blood; regulation of inflammatory response; receptor biosynthetic process; angiotensin mediated drinking behavior

48-Strip-Wells

440 USD

96-Strip-Wells

640

5x96-Strip-Wells

2895

10x96-Strip-Wells

5415