ELISA/assay

Mouse Fatty Acid Synthase ELISA Kit

MBS731124

48-Strip-Wells-(Comp

470 USD

ELISA Kit

Mouse Fatty Acid Synthase ELISA Kit

Fatty Acid Synthase

fatty acid synthase; Fatty acid synthase; fatty acid synthase; short chain dehydrogenase/reductase family 27X, member 1; fatty acid synthase

FAS

FASN; FASN; FAS; OA-519; SDR27X1; FAS

FAS_HUMAN

Mouse

This assay has high sensitivity and excellent specificity for detection of FAS. No significant cross-reactivity or interference between FAS and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FAS and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 1.0 mug/mL.

Intended Uses: This FAS ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse FAS. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the assay: FAS ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for FAS. Standards or samples are then added to the microtiter plate wells and FAS if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of FAS present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for FAS are added to each well to "sandwich" the FAS immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain FAS and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FAS concentration in each sample is interpolated from this standard curve.

41872631

NP_004095.4

NM_004104.4

273,427 Da

AMPK Signaling Pathway (198868); AMPK Signaling Pathway (989139); AMPK Signaling Pathway (992181); Activation Of Gene Expression By SREBF (SREBP) Pathway (685552); ChREBP Activates Metabolic Gene Expression Pathway (106104); Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway (906000); Defective BTD Causes Biotidinase Deficiency Pathway (906015); Defective CD320 Causes Methylmalonic Aciduria Pathway (906012); Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway (906001); Defective GIF Causes Intrinsic Factor Deficiency Pathway (906004)

The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. [provided by RefSeq, Jul 2008]

FASN: a multifunctional enzyme. Catalyzes the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. Protein type: EC 4.2.1.59; Lyase; Lipid Metabolism - fatty acid biosynthesis; EC 2.3.1.85; EC 2.3.1.41; EC 1.1.1.100; Hydrolase; Acetyltransferase; EC 2.3.1.38; EC 1.3.1.39; Oxidoreductase; EC 3.1.2.14; EC 2.3.1.39. Chromosomal Location of Human Ortholog: 17q25. Cellular Component: Golgi apparatus; membrane; mitochondrion; glycogen granule; cytoplasm; plasma membrane; melanosome; cytosol. Molecular Function: fatty-acid synthase activity; protein homodimerization activity; myristoyl-[acyl-carrier-protein] hydrolase activity; 3-oxoacyl-[acyl-carrier-protein] synthase activity; zinc ion binding; enoyl-[acyl-carrier-protein] reductase (NADPH, A-specific) activity; enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) activity; [acyl-carrier-protein] S-acetyltransferase activity; drug binding; 3-oxoacyl-[acyl-carrier-protein] reductase activity; protein binding; 3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity; oleoyl-[acyl-carrier-protein] hydrolase activity; [acyl-carrier-protein] S-malonyltransferase activity; palmitoyl-[acyl-carrier-protein] hydrolase activity; 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase activity. Biological Process: osteoblast differentiation; vitamin metabolic process; acetyl-CoA metabolic process; pantothenate metabolic process; energy reserve metabolic process; positive regulation of cellular metabolic process; triacylglycerol biosynthetic process; cellular lipid metabolic process; fatty acid metabolic process; water-soluble vitamin metabolic process; fatty acid biosynthetic process

48-Strip-Wells-(Competitive)

470 USD

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675