Human Early Growth Response Protein 1 ELISA Kit | MyBioSource MBS731077 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Early Growth Response Protein 1 ELISA Kit

catalog :

MBS731077

quantity :

48-Strip-Wells-(Comp

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS731077

products type :

ELISA Kit

products full name :

Human Early Growth Response Protein 1 ELISA Kit

products short name :

Early Growth Response Protein 1

other names :

early growth response 1, isoform CRA_b; Early growth response protein 1; early growth response protein 1; EGR-1; zinc finger protein 225; transcription factor ETR103; transcription factor Zif268; zinc finger protein Krox-24; nerve growth factor-induced protein A; early growth response 1; AT225; Nerve growth factor-induced protein A; NGFI-A; Transcription factor ETR103; Transcription factor Zif268; Zinc finger protein 225; Zinc finger protein Krox-24

products gene name :

EGR1

other gene names :

EGR1; EGR1; TIS8; AT225; G0S30; NGFI-A; ZNF225; KROX-24; ZIF-268; KROX24; ZNF225; EGR-1; NGFI-A

uniprot entry name :

EGR1_HUMAN

reactivity :

Human

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate

other info2 :

Intended Uses: This EGR-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human EGR-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications. !Intended Uses: This EGR-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human EGR-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Cancer

products description :

Principle of the Assay: EGR-1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for EGR-1. Standards or samples are then added to the microtiter plate wells and EGR-1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of EGR-1present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for EGR-1 are added to each well to "sandwich" the EGR-1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain EGR-1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The EGR-1 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

119582540

ncbi acc num :

EAW62136.1

ncbi mol weight :

57,507 Da

ncbi pathways :

BDNF Signaling Pathway (712093); Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway (137993); Cytokine Signaling In Immune System Pathway (366171); Downstream Signaling In Naive CD8+ T Cells Pathway (138018); ErbB1 Downstream Signaling Pathway (138057); Glucocorticoid Receptor Regulatory Network Pathway (138014); HTLV-I Infection Pathway (373901); HTLV-I Infection Pathway (373889); Immune System Pathway (106386); Insulin Signaling Pathway (198845)

ncbi summary :

The protein encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppresor gene. [provided by RefSeq, Jul 2008]

uniprot summary :

EGR1: Transcriptional regulator. Recognizes and binds to the DNA sequence 5 -CGCCCCCGC-3 (EGR-site). Activates the transcription of target genes whose products are required for mitogenesis and differentiation. By growth factors. Belongs to the EGR C2H2-type zinc-finger protein family. Protein type: C2H2-type zinc finger protein; DNA-binding; Transcription factor. Chromosomal Location of Human Ortholog: 5q31.1. Cellular Component: nucleoplasm; cytoplasm; nucleus. Molecular Function: histone acetyltransferase binding; protein binding; DNA binding; sequence-specific DNA binding; metal ion binding; double-stranded DNA binding; transcription factor binding; transcription factor activity. Biological Process: response to nutrient levels; circadian rhythm; regulation of long-term neuronal synaptic plasticity; transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; cytokine and chemokine mediated signaling pathway; response to amphetamine; negative regulation of transcription from RNA polymerase II promoter; response to cocaine; BMP signaling pathway; learning and/or memory; response to ethanol; cellular response to insulin stimulus; positive regulation of neuron apoptosis; response to glucose stimulus; oligodendrocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; response to electrical stimulus; negative regulation of apoptosis; T cell differentiation; regulation of protein sumoylation

size1 :

48-Strip-Wells-(Competitive)

price1 :

470 USD

size2 :

48-Strip-Wells-(Sandwich)

price2 :

470

size3 :

96-Strip-Wells-(Competitive)

price3 :

675

size4 :

96-Strip-Wells-(Sandwich)

price4 :

675

more info or order :

MyBioSource product webpage