ELISA/assay

Mouse Dopamine D1 receptor ELISA Kit

MBS731030

48-Strip-Wells

470 USD

ELISA Kit

Mouse Dopamine D1 receptor ELISA Kit

Dopamine D1 receptor

Mouse Dopamine D1 receptor ELISA Kit; Dopamine D1 receptor; Dopamine D1 receptor (Mouse)

dopamine D1 receptor; dopamine D1 receptor

D1R

D1R; DRD1

Mouse

Sensitivity: The sensitivity in this assay is 0.01ng/mL. Specificity: This assay has high sensitivity and excellent specificity for detection of D1R. No significant cross-reactivity or interference between D1R and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma

Mouse ELISA Kit

For Samples: Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma. Intended Uses: This D1R ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of D1R in the sample, this D1R ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus D1R concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve. Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of D1R utilizes a multiclonal anti-D1R antibody and an D1R-HRP conjugate. The assay sample and buffer are incubated together with D1R-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the D1R concentration since D1R from samples and D1R-HRP conjugate compete for the anti-D1R antibody binding site. Since the number of sites is limited, as more sites are occupied by D1R from the sample, fewer sites are left to bind D1R-HRP conjugate. Standards of known D1R concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of D1R. The D1R concentration in each sample is interpolated from this standard curve.

312176376

NP_001185888.1

NM_001198959.1

48-Strip-Wells

470 USD

96-Strip-Wells

675