ELISA/assay

Rat Thyroxine ELISA Kit

MBS731017

48-Strip-Wells

470 USD

ELISA Kit

Rat Thyroxine ELISA Kit

Thyroxine

T4

Rat

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ng/mL.

Intended Uses: This TT4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat TT4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: TT4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TT4 antibody and an TT4-HRP conjugate. The assay sample and buffer are incubated together with TT4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TT4 concentration since TT4 from samples and TT4-HRP conjugate compete for the anti-TT4 antibody binding site. Since the number of sites is limited, as more sites are occupied by TT4 from the sample, fewer sites are left to bind TT4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TT4 concentration in each sample is interpolated from this standard curve.

48-Strip-Wells

470 USD

96-Strip-Wells

675