ELISA/assay

Rat BCL2 Associated X Protein ELISA Kit

MBS730995

48-Strip-Wells

470 USD

ELISA Kit

Rat BCL2 Associated X Protein ELISA Kit

BCL2 Associated X Protein

bax, partial; Apoptosis regulator BAX; apoptosis regulator BAX; Bcl2-associated X protein

BAX

Bax; Bax

BAX_RAT

Rat

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0 pg/mL.

Cell Biology

Intended Uses: This BAX ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat BAX. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: BAX ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for BAX. Standards or samples are then added to the microtiter plate wells and BAX if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of BAX present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for BAX are added to each well to "sandwich" the BAX immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain BAX and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BAX concentrationin each sample is interpolated from this standard curve.

975870

AAA75200.1

Da

ATM Pathway (198524); Activation, Translocation And Oligomerization Of BAX Pathway (1010647); Amyotrophic Lateral Sclerosis (ALS) Pathway (83491); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Apoptosis Pathway (219785); Apoptosis Pathway (83452); Apoptosis Pathway (470); Apoptosis Pathway (1010630); B Cell Receptor Signaling Pathway (198486); Colorectal Cancer Pathway (83494)

Bcl2-related gene; involved in the regulation of apoptotic cell death [RGD, Feb 2006]

BAX: Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis. Homodimer. Forms higher oligomers under stress conditions. Interacts with BCL2L11. Interaction with BCL2L11 promotes BAX oligomerization and association with mitochondrial membranes, with subsequent release of cytochrome c. Forms heterodimers with BCL2, E1B 19K protein, BCL2L1 isoform Bcl-X(L), BCL2L2, MCL1 and A1. Interacts with SH3GLB1 and HN. Interacts with SFN and YWHAZ; the interaction occurs in the cytoplasm. Under stress conditions, JNK-mediated phosphorylation of SFN and YWHAZ, releases BAX to mitochondria. Isoform Sigma interacts with BCL2A1 and BCL2L1 isoform Bcl-X(L). Interacts with RNF144B, which regulates the ubiquitin-dependent stability of BAX. Interacts with CLU under stress conditions that cause a conformation change leading to BAX oligomerization and association with mitochondria. Does not interact with CLU in unstressed cells. Interacts with FAIM2/LFG2. Interacts with human cytomegalovirus/HHV-5 protein vMIA/UL37. Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro- myelocytic leukemia, histiocytic lymphoma, Burkitt s lymphoma, T- cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines. Belongs to the Bcl-2 family. 8 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Tumor suppressor; Mitochondrial; Apoptosis. Cellular Component: mitochondrial permeability transition pore complex; endoplasmic reticulum membrane; mitochondrion; cell; endoplasmic reticulum; nuclear envelope; cytosol; pore complex; mitochondrial outer membrane; membrane; mitochondrial membrane; cytoplasm; intracellular; nucleus. Molecular Function: BH domain binding; identical protein binding; protein binding; protein homodimerization activity; channel activity; protein heterodimerization activity; chaperone binding; heat shock protein binding; BH3 domain binding; protein complex binding; lipid binding. Biological Process: hypothalamus development; positive regulation of apoptosis; regulation of cell cycle; response to toxin; germ cell programmed cell death; myeloid cell homeostasis; homeostasis of number of cells; B cell apoptosis; post-embryonic development; germ cell development; regulation of mammary gland epithelial cell proliferation; spermatid differentiation; development of secondary sexual characteristics; regulation of mitochondrial membrane potential; protein insertion into mitochondrial membrane during induction of apoptosis; regulation of neuron apoptosis; establishment and/or maintenance of transmembrane electrochemical gradient; negative regulation of neuron apoptosis; negative regulation of protein binding; kidney development; response to corticosterone stimulus; response to drug; inner mitochondrial membrane organization and biogenesis; nervous system development; release of cytochrome c from mitochondria; outer mitochondrial membrane organization and biogenesis; positive regulation of B cell apoptosis; regulation of protein homodimerization activity; cellular respiration; vagina development; protein oligomerization; fertilization; DNA damage response, signal transduction resulting in induction of apoptosis; induction of apoptosis via death domain receptors; negative regulation of fibroblast proliferation; retina development in camera-type eye; reduction of endoplasmic reticulum calcium ion concentration; glycosphingolipid metabolic process; response to ionizing radiation; mitochondrial fragmentation during apoptosis; cerebral cortex development; regulation of nitrogen utilization; post-embryonic camera-type eye morphogenesis; positive regulation of pigmentation; regulation of protein heterodimerization activity; apoptosis; T cell homeostatic proliferation; negative regulation of peptidyl-serine phosphorylation; neuron migration; positive regulation of apoptosis involved in mammary gland involution; regulation of caspase activity; response to salt stress; release of matrix enzymes from mitochondria; negative regulation of cell proliferation; positive regulation of protein oligomerization; apoptotic mitochondrial changes; B cell homeostatic proliferation; ovarian follicle development; B cell homeostasis; positive regulation of neuron apoptosis; response to wounding; response to gamma radiation; response to axon injury; B cell negative selection; protein homooligomerization; leukocyte homeostasis; caspase activation; mitochondrial fusion; transformed cell apoptosis; male gonad development; Sertoli cell proliferation; response to cocaine; limb morphogenesis; odontogenesis of dentine-containing teeth; cell proliferation; neuron apoptosis; response to copper ion; homeostasis of number of cells within a tissue; blood vessel remodeling; retinal cell programmed cell death; spermatogenesis; brain development; positive regulation of release of sequestered calcium ion into cytosol; caspase activation via cytochrome c; sex differentiation; response to DNA damage stimulus

48-Strip-Wells

470 USD

96-Strip-Wells

675