ELISA/assay

Rat Glucocorticoid receptor-alpha ELISA Kit

MBS730987

48-Strip-Wells

470 USD

ELISA Kit

Rat Glucocorticoid receptor-alpha ELISA Kit

Glucocorticoid receptor-alpha

Rat Glucocorticoid receptor-a ELISA Kit

glucocorticoid receptor isoform alpha

GR-alpha

GR-a

Rat

This assay has high sensitivity and excellent specificity for detection of GRalpha. No significant cross-reactivity or interference between GRalpha and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GRalpha and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This GRalpha ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat GRalpha. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Signal Transduction

. Principle of the assay: GRalpha ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GRalpha antibody and an GRalpha-HRP conjugate. The assay sample and buffer are incubated together with GRalpha-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GRalpha concentration since GRalpha from samples and GRalpha-HRP conjugate compete for the anti-GRalpha antibody binding site. Since the number of sites is limited, as more sites are occupied by GRalpha from the sample, fewer sites are left to bind GRalpha-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GRalpha concentration in each sample is interpolated from this standard curve.

4504133

NP_000167.1

NM_000176.2

Adipogenesis Pathway (198832); BMAL1:CLOCK,NPAS2 Activates Circadian Gene Expression Pathway (477138); Circadian Clock Pathway (187173); FOXA2 And FOXA3 Transcription Factor Networks Pathway (137911); Gene Expression Pathway (105937); Generic Transcription Pathway (105938); Glucocorticoid Receptor Regulatory Network Pathway (138014); Neuroactive Ligand-receptor Interaction Pathway (83053); Neuroactive Ligand-receptor Interaction Pathway (462); Nuclear Receptor Transcription Pathway (105979)

This gene encodes glucocorticoid receptor, which can function both as a transcription factor that binds to glucocorticoid response elements in the promoters of glucocorticoid responsive genes to activate their transcription, and as a regulator of other transcription factors. This receptor is typically found in the cytoplasm, but upon ligand binding, is transported into the nucleus. It is involved in inflammatory responses, cellular proliferation, and differentiation in target tissues. Mutations in this gene are associated with generalized glucocorticoid resistance. Alternative splicing of this gene results in transcript variants encoding either the same or different isoforms. Additional isoforms resulting from the use of alternate in-frame translation initiation sites have also been described, and shown to be functional, displaying diverse cytoplasm-to-nucleus trafficking patterns and distinct transcriptional activities (PMID:15866175). [provided by RefSeq, Feb 2011]

GR: transcription factor of the nuclear receptor family. Receptor for glucocorticoids (GC). Binds to glucocorticoid response elements (GRE) and modulates other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Three alternatively spliced isoforms have been described. Protein type: Transcription factor; DNA-binding; Nuclear receptor; Mitochondrial. Chromosomal Location of Human Ortholog: 5q31.3. Cellular Component: nucleoplasm; membrane; mitochondrial matrix; cytoplasm; cytosol; nucleus. Molecular Function: protein dimerization activity; protein binding; glucocorticoid receptor activity; zinc ion binding; transcription factor activity; steroid binding. Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; adrenal gland development; maternal behavior; regulation of glucocorticoid biosynthetic process; chromatin modification; signal transduction; glucocorticoid metabolic process; glucocorticoid mediated signaling; regulation of transcription, DNA-dependent; positive regulation of neuron apoptosis; glucocorticoid receptor signaling pathway; gene expression; regulation of gluconeogenesis; positive regulation of transcription from RNA polymerase II promoter. Disease: Glucocorticoid Resistance, Generalized

48-Strip-Wells

470 USD

96-Strip-Wells

675