catalog number :
MBS730907
products type :
ELISA Kit
products full name :
Human Anti Diuretic Hormone ELISA Kit
products short name :
Anti Diuretic Hormone
other names :
ADH; Alcohol dehydrogenase
other gene names :
Adh; CG3481
uniprot entry name :
ADH_DROME
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 1.0 pg/mL.
other info2 :
Intended Uses: This AVP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human AVP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products description :
Principle of the Assay: AVP ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for AVP. Standards or samples are then added to the microtiter plate wells and AVP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of AVP present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for AVP are added to each well to "sandwich" the AVP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain AVP and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AVP concentration in each sample is interpolated from this standard curve.
ncbi acc num :
CAA77330.1
ncbi mol weight :
27,761 Da
size1 :
48-Strip-Wells-(Competitive)
size2 :
48-Strip-Wells-(Sandwich)
size3 :
96-Strip-Wells-(Competitive)
size4 :
96-Strip-Wells-(Sandwich)