ELISA/assay

Mouse Apolipoprotein C3 ELISA Kit

MBS730871

48-Strip-Wells

470 USD

ELISA Kit

Mouse Apolipoprotein C3 ELISA Kit

Apolipoprotein C3

apolipoprotein C-III; Apolipoprotein C-III; apolipoprotein C-III; apo-CIII; apoC-III; apolipoprotein C3; apolipoprotein C-III; Apolipoprotein C3

APOC3

APOC3; APOC3; HALP2; APOCIII; Apo-CIII; ApoC-III

APOC3_HUMAN

Mouse

This assay has high sensitivity and excellent specificity for detection of Apo-C3. No significant cross-reactivity or interference between Apo-C3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between Apo-C3 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0 ng/mL.

Intended Uses: This Apo-C3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse Apo-C3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cardiovascular

Principle of the assay: Apo-C3 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for Apo-C3. Standards or samples are then added to the microtiter plate wells and Apo-C3 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of Apo-C3 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for Apo-C3 are added to each well to “sandwich” the Apo-C3 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain Apo-C3 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Apo-C3 concentration in each sample is interpolated from this standard curve.

4557323

NP_000031.1

NM_000040.1

10,852 Da

Chylomicron-mediated Lipid Transport Pathway (106157); Disease Pathway (530764); Diseases Associated With Visual Transduction Pathway (771581); HDL-mediated Lipid Transport Pathway (106158); Lipid Digestion, Mobilization, And Transport Pathway (106111); Lipoprotein Metabolism Pathway (106156); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); PPAR Signaling Pathway (83042); PPAR Signaling Pathway (450)

Apolipoprotein C-III is a very low density lipoprotein (VLDL) protein. APOC3 inhibits lipoprotein lipase and hepatic lipase; it is thought to delay catabolism of triglyceride-rich particles. The APOA1, APOC3 and APOA4 genes are closely linked in both rat and human genomes. The A-I and A-IV genes are transcribed from the same strand, while the A-1 and C-III genes are convergently transcribed. An increase in apoC-III levels induces the development of hypertriglyceridemia. [provided by RefSeq, Jul 2008]

APOC3: Inhibits lipoprotein lipase and hepatic lipase and decreases the uptake of lymph chylomicrons by hepatic cells. This suggests that it delays the catabolism of triglyceride-rich particles. Defects in APOC3 are the cause of hyperalphalipoproteinemia type 2 (HALP2). HALP2 is a condition characterized by high levels of high density lipoprotein (HDL) and increased HDL cholesterol levels. Belongs to the apolipoprotein C3 family. Protein type: Lipid-binding; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 11q23.3. Cellular Component: extracellular space; chylomicron; early endosome; extracellular region. Molecular Function: lipase inhibitor activity; cholesterol binding; phospholipid binding; enzyme regulator activity. Biological Process: response to drug; response to peptide hormone stimulus; phototransduction, visible light; cholesterol metabolic process; regulation of Cdc42 protein signal transduction; cholesterol efflux; lipoprotein metabolic process; G-protein coupled receptor protein signaling pathway; cholesterol homeostasis; triacylglycerol metabolic process; reverse cholesterol transport; phospholipid efflux; negative regulation of lipid catabolic process; lipoprotein transport; triacylglycerol catabolic process; negative regulation of receptor-mediated endocytosis; negative regulation of lipoprotein lipase activity; negative regulation of lipid metabolic process; triacylglycerol mobilization; inflammatory response; retinoid metabolic process; negative regulation of fatty acid biosynthetic process; response to nutrient. Disease: Apolipoprotein C-iii Deficiency

48-Strip-Wells

470 USD

96-Strip-Wells

675