ELISA/assay

Guinea Pig amyloid beta peptide 1-40 ELISA Kit

MBS730736

48-Strip-Wells

470 USD

ELISA Kit

Guinea Pig amyloid beta peptide 1-40 ELISA Kit

amyloid beta peptide 1-40

Guinea Pig amyloid beta peptide 1-40 ELISA Kit; amyloid beta peptide 1-40; amyloid beta peptide 1-40 (Guinea Pig)

Abeta1-40

Ab1-40; A 1-40

GuineaPig

This assay has high sensitivity and excellent specificity for detection of Abeta1~40. No significant cross-reactivity or interference between Abeta1~40 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between Abeta1~40 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Intended Uses: This Abeta1~40 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea pig Abeta1~40. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Guinea Pig ELISA Kit

Principle of the assay: Abeta1~40 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Abeta1~40 antibody and an Abeta1~40-HRP conjugate. The assay sample and buffer are incubated together with Abeta1~40-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Abeta1~40 concentration since Abeta1~40 from samples and Abeta1~40-HRP conjugate compete for the anti-Abeta1~40 antibody binding site. Since the number of sites is limited, as more sites are occupied by Abeta1~40 from the sample, fewer sites are left to bind Abeta1~40-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Abeta1~40 concentration in each sample is interpolated from this standard curve.

48-Strip-Wells

470 USD

96-Strip-Wells

675