ELISA/assay

Human 12 Lipoxygenase ELISA Kit

MBS730723

48-Strip-Wells-(Comp

470 USD

ELISA Kit

Human 12 Lipoxygenase ELISA Kit

12 Lipoxygenase

12-lipoxygenase, partial; Arachidonate 15-lipoxygenase; arachidonate 15-lipoxygenase; 15-LOX; 12/15-lipoxygenase; 15-lipooxygenase-1; arachidonate omega-6 lipoxygenase; arachidonate 12-lipoxygenase, leukocyte-type; arachidonate 15-lipoxygenase; 12/15-lipoxygenase; Arachidonate 12-lipoxygenase, leukocyte-type (EC:1.13.11.31); 12-LOX; Arachidonate omega-6 lipoxygenase

LOX 12

ALOX15; ALOX15; 12-LOX; 15LOX-1; 15-LOX-1; LOG15; 15-LOX; 15-LOX-1; 12-LOX

LOX15_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of LOX12. No significant cross-reactivity or interference between LOX12 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between LOX12 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Sensitivity: 0.1 ng/mL.

Intended Uses: This LOX12 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human LOX12. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay LOX12 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for LOX12. Standards or samples are then added to the microtiter plate wells and LOX12 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of LOX12 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for LOX12 are added to each well to "sandwich" the LOX12 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain LOX12 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LOX12 concentration in each sample is interpolated from this standard curve.

553159

AAA35482.1

74,804 Da

Arachidonic Acid Metabolism Pathway (82991); Arachidonic Acid Metabolism Pathway (685553); Arachidonic Acid Metabolism Pathway (366); Eicosanoid Synthesis Pathway (198888); IL4-mediated Signaling Events Pathway (137933); Linoleic Acid Metabolism Pathway (82992); Linoleic Acid Metabolism Pathway (367); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976)

ALOX15: Converts arachidonic acid to 15S- hydroperoxyeicosatetraenoic acid. Also acts on C-12 of arachidonate as well as on linoleic acid. Belongs to the lipoxygenase family. Protein type: EC 1.13.11.33; Lipid Metabolism - linoleic acid; Oxidoreductase; Lipid Metabolism - arachidonic acid; EC 1.13.11.31; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 17p13.3. Cellular Component: extrinsic to internal side of plasma membrane; membrane; plasma membrane; lipid particle; cytosol. Molecular Function: protein binding; phosphatidylinositol-4,5-bisphosphate binding; hepoxilin A3 synthase activity; arachidonate 12-lipoxygenase activity; arachidonate 15-lipoxygenase activity; hepoxilin-epoxide hydrolase activity; iron ion binding. Biological Process: ossification; positive regulation of heterotypic cell-cell adhesion; wound healing; positive regulation of actin filament polymerization; lipoxygenase pathway; negative regulation of adaptive immune response; phosphatidylethanolamine biosynthetic process; arachidonic acid metabolic process; leukotriene metabolic process; hepoxilin biosynthetic process; inflammatory response; apoptotic cell clearance; bone mineralization

48-Strip-Wells-(Competitive)

470 USD

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675