ELISA/assay

Mouse Ornithine Carbamoyl Transferase ELISA Kit

MBS730631

48-Strip-Wells

470 USD

ELISA Kit

Mouse Ornithine Carbamoyl Transferase ELISA Kit

Ornithine Carbamoyl Transferase

ornithine carbamoyl transferase; Ornithine carbamoyltransferase; ornithine carbamoyl transferase

OCT

argF; argF; BBB_1085; OTCase

I3WIG4_BIFBI

Mouse

This assay has high sensitivity and excellent specificity for detection of OCT. No significant cross-reactivity or interference between OCT and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between OCT and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Intended Uses: This OCT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse OCT. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the assay: OCT ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-OCT antibody and an OCT-HRP conjugate. The assay sample and buffer are incubated together with OCT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the OCT concentration since OCT from samples and OCT-HRP conjugate compete for the anti-OCT antibody binding site. Since the number of sites is limited, as more sites are occupied by OCT from the sample, fewer sites are left to bind OCT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OCT concentration in each sample is interpolated from this standard curve.

390936997

YP_006394556.1

NC_017999.1

35,049 Da

Arginine And Proline Metabolism Pathway (657556); Arginine And Proline Metabolism Pathway (323); Biosynthesis Of Amino Acids Pathway (792178); Biosynthesis Of Amino Acids Pathway (795174); Biosynthesis Of Secondary Metabolites Pathway (657600); Metabolic Pathways (657599)

48-Strip-Wells

470 USD

96-Strip-Wells

675