catalog number :
MBS730610
products type :
ELISA Kit
products full name :
Mouse Non-ester Fatty Acid ELISA Kit
products short name :
Non-ester Fatty Acid
other names :
neuraminidase; Neuraminidase
products gene name :
NEFA
uniprot entry name :
NRAM_I57A1
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 umol/L.
other info2 :
Intended Uses: This nFA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse nFA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products description :
Principle of the Assay: nFA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-nFA antibody and an nFA-HRP conjugate. The assay sample and buffer are incubated together with nFA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the nFA concentration since nFA from samples and nFA-HRP conjugate compete for the anti-nFA antibody binding site. Since the number of sites is limited, as more sites are occupied by nFA from the sample, fewer sites are left to bind nFA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The nFA concentration in each sample is interpolated from this standard curve.
ncbi acc num :
AAA96709.2
ncbi mol weight :
51,888 Da