Rat Tissue factor pathway inhibitor ELISA Kit | MyBioSource MBS730482 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Tissue factor pathway inhibitor ELISA Kit

catalog :

MBS730482

quantity :

48-Strip-Wells-(Comp

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS730482

products type :

ELISA Kit

products full name :

Rat Tissue factor pathway inhibitor ELISA Kit

products short name :

Tissue factor pathway inhibitor

other names :

tissue factor pathway inhibitor isoform b; Tissue factor pathway inhibitor; tissue factor pathway inhibitor; anti-convertin; extrinsic pathway inhibitor; tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor); Extrinsic pathway inhibitor; EPI; Lipoprotein-associated coagulation inhibitor; LACI

products gene name :

TFPI

other gene names :

TFPI; TFPI; EPI; TFI; LACI; TFPI1; LACI; TFPI1; TFPI; EPI; LACI

uniprot entry name :

TFPI1_HUMAN

reactivity :

Rat

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 0.1 ng/mL.

other info2 :

Intended Uses: This TFPI ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat TFPI. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Cardiovascular

products description :

Principle of the Assay: TFPI ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TFPI antibody and an TFPI-HRP conjugate. The assay sample and buffer are incubated together with TFPI-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TFPI concentration since TFPI from samples and TFPI-HRP conjugate compete for the anti-TFPI antibody binding site. Since the number of sites is limited, as more sites are occupied by TFPI from the sample, fewer sites are left to bind TFPI-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TFPI concentration in each sample is interpolated from this standard curve.

ncbi gi num :

73760409

ncbi acc num :

NP_001027452.1

ncbi gb acc num :

NM_001032281.2

ncbi mol weight :

28,653 Da

ncbi pathways :

Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Extrinsic Pathway (106058); Formation Of Fibrin Clot (Clotting Cascade) Pathway (106057); Hemostasis Pathway (106028); Integrated Breast Cancer Pathway (219801); Syndecan-4-mediated Signaling Events Pathway (138064)

ncbi summary :

This gene encodes a protease inhibitor that regulates the tissue factor (TF)-dependent pathway of blood coagulation. The coagulation process initiates with the formation of a factor VIIa-TF complex, which proteolytically activates additional proteases (factors IX and X) and ultimately leads to the formation of a fibrin clot. The product of this gene inhibits the activated factor X and VIIa-TF proteases in an autoregulatory loop. The encoded protein is glycosylated and predominantly found in the vascular endothelium and plasma in both free forms and complexed with plasma lipoproteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been confirmed. [provided by RefSeq, Jul 2008]

uniprot summary :

TFPI: Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, GPI anchor; Secreted, signal peptide; Inhibitor; Secreted. Chromosomal Location of Human Ortholog: 2q32. Cellular Component: extracellular space; cell surface; endoplasmic reticulum; plasma membrane; extracellular region. Molecular Function: serine-type endopeptidase inhibitor activity; endopeptidase inhibitor activity. Biological Process: blood coagulation, extrinsic pathway; blood coagulation

size1 :

48-Strip-Wells-(Competitive)

price1 :

470 USD

size2 :

48-Strip-Wells-(Sandwich)

price2 :

470

size3 :

96-Strip-Wells-(Competitive)

price3 :

675

size4 :

96-Strip-Wells-(Sandwich)

price4 :

675

more info or order :

MyBioSource product webpage