ELISA/assay

Mouse Phospho protein kinase C ELISA Kit

MBS730452

48-Strip-Wells

470 USD

ELISA Kit

Mouse Phospho protein kinase C ELISA Kit

Phospho protein kinase C

P-PKC

Mouse

Store all reagents at 2-8 degree C.

Samples: Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma

For Samples: Cell culture fluid & body fluid & tissue homogenate serum or blood plasma. Intended Uses: This pPKC ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of pPKC in the sample, this pPKC ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus pPKC concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve. Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of pPKC utilizes a polyclonal anti-pPKC antibody and an pPKC-HRP conjugate. The assay sample and buffer are incubated together with pPKC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the pPKC concentration since pPKC from samples and pPKC-HRP conjugate compete for the anti-pPKC antibody binding site. Since the number of sites is limited, as more sites are occupied by pPKC from the sample, fewer sites are left to bind pPKC-HRP conjugate. Standards of known pPKC concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (O.D.) to the concentration of pPKC. The pPKC concentration in each sample is interpolated from this standard curve.

48-Strip-Wells

470 USD

96-Strip-Wells

675