ELISA/assay

Human Activin Receptor Like Kinase 1 ELISA Kit

MBS730439

48-Strip-Wells

470 USD

ELISA Kit

Human Activin Receptor Like Kinase 1 ELISA Kit

Activin Receptor Like Kinase 1

activin receptor like kinase 1; Serine/threonine-protein kinase receptor R3; serine/threonine-protein kinase receptor R3; TGF-B superfamily receptor type I; activin A receptor, type II-like kinase 1; activin A receptor type II-like 1; Activin receptor-like kinase 1; ALK-1; TGF-B superfamily receptor type I; TSR-I

ALK1

ACVRL1; ACVRL1; HHT; ALK1; HHT2; ORW2; SKR3; ALK-1; TSR-I; ACVRLK1; ACVRLK1; ALK1; SKR3; ALK-1; TSR-I

ACVL1_HUMAN

Human

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich

Signal Transduction

For Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Intended Uses: This ALK1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ALK1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: ALK1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ALK1. Standards or samples are then added to the microtiter plate wells and ALK1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ALK1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ALK1 are added to each well to "sandwich" the ALK1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ALK1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ALK1 concentration in each sample is interpolated from this standard curve.

2228562

AAB61900.1

56,124 Da

ALK1 Pathway (137985); ALK1 Signaling Events Pathway (137968); Id Signaling Pathway (198871); TGF-beta Receptor Signaling Pathway (198774)

This gene encodes a type I cell-surface receptor for the TGF-beta superfamily of ligands. It shares with other type I receptors a high degree of similarity in serine-threonine kinase subdomains, a glycine- and serine-rich region (called the GS domain) preceding the kinase domain, and a short C-terminal tail. The encoded protein, sometimes termed ALK1, shares similar domain structures with other closely related ALK or activin receptor-like kinase proteins that form a subfamily of receptor serine/threonine kinases. Mutations in this gene are associated with hemorrhagic telangiectasia type 2, also known as Rendu-Osler-Weber syndrome 2. [provided by RefSeq, Jul 2008]

ALK1: a kinase of the STKR family. A type I cell-surface receptor for the TGF-beta superfamily of ligands including Activin A. Highly expressed in human placenta and lung. Deficiency causes hemorrhagic telangiectasia type 2 also known as Rendu-Osler-Weber syndrome 2. Protein type: Protein kinase, TKL; Protein kinase, Ser/Thr (receptor); Membrane protein, integral; Kinase, protein; EC 2.7.11.30; TKL group; STKR family; Type1 subfamily. Chromosomal Location of Human Ortholog: 12q13.13. Cellular Component: cell surface; cell soma; integral to plasma membrane; dendrite. Molecular Function: transforming growth factor beta receptor activity; protein serine/threonine kinase activity; protein binding; activin receptor activity, type I; transforming growth factor beta receptor activity, type I; transforming growth factor beta binding; metal ion binding; activin binding; SMAD binding; protein kinase binding; transmembrane receptor protein serine/threonine kinase activity; ATP binding; receptor signaling protein serine/threonine kinase activity. Biological Process: protein heterooligomerization; blood circulation; positive regulation of transcription, DNA-dependent; activin receptor signaling pathway; negative regulation of cell adhesion; regulation of blood vessel endothelial cell migration; signal transduction; protein amino acid phosphorylation; BMP signaling pathway; negative regulation of cell proliferation; positive regulation of endothelial cell differentiation; negative regulation of endothelial cell differentiation; regulation of transcription, DNA-dependent; regulation of blood pressure; transforming growth factor beta receptor signaling pathway; lymphangiogenesis; angiogenesis; negative regulation of cell migration; positive regulation of BMP signaling pathway; regulation of DNA replication; regulation of endothelial cell proliferation; in utero embryonic development; negative regulation of focal adhesion formation; negative regulation of blood vessel endothelial cell migration; blood vessel maturation; blood vessel endothelial cell proliferation during sprouting angiogenesis; positive regulation of angiogenesis; positive regulation of chondrocyte differentiation; negative regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of endothelial cell proliferation; blood vessel remodeling; negative regulation of cell growth; wound healing, spreading of epidermal cells. Disease: Telangiectasia, Hereditary Hemorrhagic, Type 2

48-Strip-Wells

470 USD

96-Strip-Wells

675