ELISA/assay

Mouse Axis Inhibition Protein 2 ELISA Kit

MBS730416

48-Strip-Wells

470 USD

ELISA Kit

Mouse Axis Inhibition Protein 2 ELISA Kit

Axis Inhibition Protein 2

AXIN2; Axin-2; axin-2; conductin; axin-like protein; axis inhibition protein 2; axin 2; Axin-like protein; Axil; Axis inhibition protein 2; Conductin

AXIN2

AXIN2; AXIN2; AXIL; ODCRCS; Axil

AXIN2_HUMAN

Mouse

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture superna tants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0 pg/mL.

Neurobiology

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate . INTENDED USE This AXIN2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse AXIN2. This ELISA kit for research use only, not for therapeutic or diagnostic applications! . PRINCIPLE OF THE ASSAY AXIN2 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for AXIN2. Standards or samples are then added to the microtiter plate wells and AXIN2 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of AXIN2 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for AXIN2 are added to each well to “sandwich” the AXIN2 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain AXIN2 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AXIN2 concentration in each sample is interpolated from this standard curve.

6653584

AAF22799.1

93,558 Da

Basal Cell Carcinoma Pathway (83113); Basal Cell Carcinoma Pathway (525); Canonical Wnt Signaling Pathway (138032); Colorectal Cancer Pathway (83106); Colorectal Cancer Pathway (518); Disease Pathway (530764); Endometrial Cancer Pathway (83109); Endometrial Cancer Pathway (521); Hippo Signaling Pathway (749777); Hippo Signaling Pathway (750388)

The Axin-related protein, Axin2, presumably plays an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway, like its rodent homologs, mouse conductin/rat axil. In mouse, conductin organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and conductin, which leads to the degradation of beta-catenin. Apparently, the deregulation of beta-catenin is an important event in the genesis of a number of malignancies. The AXIN2 gene has been mapped to 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Mutations in this gene have been associated with colorectal cancer with defective mismatch repair. [provided by RefSeq, Jul 2008]

axin 2: is a negative regulator of the Wnt pathway, which is critical in stem cell signaling, morphogenesis, the mesenchymal-epithelial transition, and many cancers. Axin functions as a tumor suppressor. Probably facilitates the phosphorylation of beta-catenin and APC by GSK3B, leading to their ubiquitination and subsequent proteolysis. Is downregulated during progression of esophageal squamous cell carcinoma. Axin1/2 mediate cross-talk between TGF-beta and Wnt signaling pathways. Protein type: Adaptor/scaffold. Chromosomal Location of Human Ortholog: 17q24.1. Cellular Component: microtubule cytoskeleton; nucleoplasm; Golgi apparatus; centrosome; cytoplasmic microtubule; cytoplasm; postsynaptic density; cytoplasmic membrane-bound vesicle; plasma membrane; cell cortex; beta-catenin destruction complex; nucleus; cytosol. Molecular Function: protein binding; enzyme binding; ubiquitin protein ligase binding; beta-catenin binding; protein kinase binding; GTPase activator activity. Biological Process: odontogenesis; negative regulation of cell proliferation; cell proliferation; intramembranous ossification; regulation of mismatch repair; mRNA stabilization; dorsal/ventral axis specification; negative regulation of osteoblast differentiation; positive regulation of protein amino acid phosphorylation; maintenance of DNA repeat elements; bone mineralization; positive regulation of GTPase activity. Disease: Oligodontia-colorectal Cancer Syndrome

48-Strip-Wells

470 USD

96-Strip-Wells

675