ELISA/assay

Rat Agouti Related Protein ELISA Kit

MBS730302

48-Strip-Wells

470 USD

ELISA Kit

Rat Agouti Related Protein ELISA Kit

Agouti Related Protein

agouti-related protein; Agouti-related protein; agouti-related protein; agouti related protein homolog; agouti related neuropeptide

AGRP

AGRP; AGRP; ART; AGRT; ASIP2; AGRT; ART

AGRP_HUMAN

Rat

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Highest Standards: 1000pg/mL. Concentration of Standards: Concentration of Standards: 0,50,100,250,500,1000pg/mL

Cancer

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate . INTENDED USE This AGRP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat AGRP. This ELISA kit for research use only, not for therapeutic or diagnostic applications! . PRINCIPLE OF THE ASSAY AGRP ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for AGRP. Standards or samples are then added to the microtiter plate wells and AGRP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of AGRP present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for AGRP are added to each well to “sandwich” the AGRP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain AGRP and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AGRP concentration in each sample is interpolated from this standard curve.

4501995

NP_001129.1

NM_001138.1

14,440 Da

Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); Syndecan-3-mediated Signaling Events Pathway (137952)

This gene encodes an antagonist of the melanocortin-3 and melanocortin-4 receptor. It appears to regulate hypothalamic control of feeding behavior via melanocortin receptor and/or intracellular calcium regulation, and thus plays a role in weight homeostasis. Mutations in this gene have been associated with late on-set obesity. [provided by RefSeq, Dec 2009]

AGRP: Plays a role in weight homeostasis. Involved in the control of feeding behavior through the central melanocortin system. Acts as alpha melanocyte-stimulating hormone antagonist by inhibiting cAMP production mediated by stimulation of melanocortin receptors within the hypothalamus and adrenal gland. Has very low activity with MC5R. Is an inverse agonist for MC3R and MC4R being able to suppress their constitutive activity. It promotes MC3R and MC4R endocytosis in an arrestin-dependent manner. Genetic variations in AGRP may be a cause of obesity (OBESITY). It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. Protein type: Secreted; Secreted, signal peptide; Hormone. Chromosomal Location of Human Ortholog: 16q22. Cellular Component: extracellular space; cell soma; Golgi lumen. Molecular Function: neuropeptide hormone activity; receptor binding. Biological Process: maternal process involved in pregnancy; hormone-mediated signaling; eating behavior; neuropeptide signaling pathway; photoperiodism; adult feeding behavior; feeding behavior; response to insulin stimulus. Disease: Obesity

48-Strip-Wells

470 USD

96-Strip-Wells

675