ELISA/assay

Human Taxilin Alpha /IL 14 ELISA Kit

MBS730250

48-Strip-Wells-(Comp

470 USD

ELISA Kit

Human Taxilin Alpha /IL 14 ELISA Kit

Taxilin Alpha /IL 14

Alpha-taxilin

TXLNa

TXLNA; TXLN

TXLNA_HUMAN

Human

Store all reagents at 2-8 degree C.

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 0.1 ng/mL.

Intended Uses: This TXLNa ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human TXLNa. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Immunology

Principle of the Assay: TXLNa ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for TXLNa. Standards or samples are then added to the microtiter plate wells and TXLNa if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of TXLNa present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for TXLNa are added to each well to "sandwich" the TXLNa immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain TXLNa and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TXLNa concentration in each sample is interpolated from this standard curve.

56417862

CAI22055.1

P40222

61,891 Da

TXLNA: May be involved in intracellular vesicle traffic and potentially in calcium-dependent exocytosis in neuroendocrine cells. Belongs to the taxilin family. Protein type: Vesicle. Chromosomal Location of Human Ortholog: 1p35.1. Cellular Component: membrane; cytoplasm; extracellular region. Molecular Function: high molecular weight B cell growth factor receptor binding; protein binding; syntaxin binding; cytokine activity. Biological Process: cell proliferation; exocytosis; B cell activation

48-Strip-Wells-(Competitive)

470 USD

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675