ELISA/assay

Mouse Alpha-fetoprotein ELISA Kit

MBS730245

48-Strip-Wells

470 USD

ELISA Kit

Mouse Alpha-fetoprotein ELISA Kit

Alpha-fetoprotein

alpha-fetoprotein; Alpha-fetoprotein; alpha-fetoprotein; alpha-fetoglobulin; alpha-1-fetoprotein; alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-fetoglobulin

AFP

AFP; AFP; AFPD; FETA; HPAFP; HPAFP

FETA_HUMAN

Mouse

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This AFP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse AFP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cancer

Principle of the Assay: AFP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AFP antibody and an AFP-HRP conjugate. The assay sample and buffer are incubated together with AFP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AFP concentration since AFP from samples and AFP-HRP conjugate compete for the anti-AFP antibody binding site. Since the number of sites is limited, as more sites are occupied by AFP from the sample, fewer sites are left to bind AFP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AFP concentration in each sample is interpolated from this standard curve.

4501989

NP_001125.1

NM_001134.2

68,678 Da

Direct P53 Effectors Pathway (137939); FOXA2 And FOXA3 Transcription Factor Networks Pathway (137911); Glucocorticoid Receptor Regulatory Network Pathway (138014); Hippo Signaling Pathway (749777); Hippo Signaling Pathway (750388)

This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Jul 2008]

AFP: Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. Dimeric and trimeric forms have been found in addition to the monomeric form. Plasma. Synthesized by the fetal liver and yolk sac. Belongs to the ALB/AFP/VDB family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 4q13.3. Cellular Component: extracellular space; cytoplasm. Molecular Function: metal ion binding. Biological Process: transport; progesterone metabolic process; ovulation from ovarian follicle. Disease: Alpha-fetoprotein Deficiency; Alpha-fetoprotein, Hereditary Persistence Of

48-Strip-Wells

470 USD

96-Strip-Wells

675