ELISA/assay

Mouse S100 Calcium Binding Protein A9 ELISA Kit

MBS730232

48-Strip-Wells

470 USD

ELISA Kit

Mouse S100 Calcium Binding Protein A9 ELISA Kit

S100 Calcium Binding Protein A9

S100A9, partial; Protein S100-A9; Calgranulin-B; Calprotectin L1H subunit; Leukocyte L1 complex heavy chain; Migration inhibitory factor-related protein 14; MRP-14; p14; S100 calcium-binding protein A9

S100A9

S100A9; CAGB; CFAG; MRP14; MRP-14; p14

S10A9_HUMAN

Mouse

This assay has high sensitivity and excellent specificity for detection of S100A9. No significant cross-reactivity or interference between S100A9 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between S100A9 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Signal Transduction

Intended Uses This S100A9 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse S100A9. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: S100A9 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-S100A9 antibody and an S100A9-HRP conjugate. The assay sample and buffer are incubated together with S100A9-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the S100A9 concentration since S100A9 from samples and S100A9-HRP conjugate compete for the anti-S100A9 antibody binding site. Since the number of sites is limited, as more sites are occupied by S100A9 from the sample, fewer sites are left to bind S100A9-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The S100A9 concentration in each sample is interpolated from this standard curve.

49457442

CAG47020.1

P06702

13,242 Da

S100A9: a calcium-binding regulatory protein of the S-100 family expressed by macrophages in acutely inflammated tissues and in chronic inflammations. May be an inhibitor of protein kinases. Also expressed in epithelial cells constitutively or induced during dermatoses. May interact with components of the intermediate filaments in monocytes and epithelial cells. Interacts with CEACAM3 in a calcium-dependent manner. Protein type: Calcium-binding. Chromosomal Location of Human Ortholog: 1q21. Cellular Component: extracellular space; cytoskeleton; extracellular region; plasma membrane; cytosol; nucleus. Molecular Function: antioxidant activity; arachidonic acid binding; signal transducer activity; RAGE receptor binding; protein binding; zinc ion binding; microtubule binding; calcium ion binding. Biological Process: caspase activation; neutrophil chemotaxis; chemokine production; chronic inflammatory response; cytokine production; response to lipopolysaccharide; positive regulation of peptide secretion; signal transduction; positive regulation of cell growth; leukocyte migration during inflammatory response; activation of NF-kappaB transcription factor; sequestering of zinc ion; response to ethanol; cell-cell signaling; response to zinc ion; actin cytoskeleton reorganization; defense response to bacterium; autophagy; innate immune response; regulation of integrin biosynthetic process; inflammatory response; defense response to fungus; regulation of cytoskeleton organization and biogenesis; positive regulation of inflammatory response

48-Strip-Wells

470 USD

96-Strip-Wells

675