Rat Dopamine decarboxylase ELISA Kit | MyBioSource MBS730226 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Dopamine decarboxylase ELISA Kit

catalog :

MBS730226

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS730226

products type :

ELISA Kit

products full name :

Rat Dopamine decarboxylase ELISA Kit

products short name :

Dopamine decarboxylase

other names :

DDC; DDC protein; aromatic-L-amino-acid decarboxylase; dopa decarboxylase (aromatic L-amino acid decarboxylase); DDC protein

products gene name :

DDC

other gene names :

DDC; DDC; AADC

uniprot entry name :

Q6IBS8_HUMAN

reactivity :

Rat

specificity :

This assay has high sensitivity and excellent specificity for detection of DDT. No significant cross-reactivity or interference between DDT and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between DDT and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ?g/mL.

other info2 :

Intended Uses: This DDT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat DDT. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Neurobiology

products description :

Principle of the assay: DDT ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DDT antibody and an DDT-HRP conjugate. The assay sample and buffer are incubated together with DDT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DDT concentration since DDT from samples and DDT-HRP conjugate compete for the anti-DDT antibody binding site. Since the number of sites is limited, as more sites are occupied by DDT from the sample, fewer sites are left to bind DDT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DDT concentration in each sample is interpolated from this standard curve.

ncbi gi num :

48145565

ncbi acc num :

CAG33005.1

uniprot acc num :

Q6IBS8

ncbi mol weight :

53,880 Da

ncbi pathways :

Alcoholism Pathway (585563); Alcoholism Pathway (587116); Amine-derived Hormones Pathway (160983); Amphetamine Addiction Pathway (547607); Amphetamine Addiction Pathway (550546); Biogenic Amine Synthesis Pathway (198793); Catecholamine Biosynthesis Pathway (160984); Catecholamine Biosynthesis, Tyrosine = Dopamine = Noradrenaline = Adrenaline Pathway (413357); Catecholamine Biosynthesis, Tyrosine = Dopamine = Noradrenaline = Adrenaline Pathway (468235); Cocaine Addiction Pathway (546258)

ncbi summary :

The encoded protein catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine. Defects in this gene are the cause of aromatic L-amino-acid decarboxylase deficiency (AADCD). AADCD deficiency is an inborn error in neurotransmitter metabolism that leads to combined serotonin and catecholamine deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jun 2011]

uniprot summary :

DDC: Catalyzes the decarboxylation of L-3,4- dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine. Homodimer. Belongs to the group II decarboxylase family. Protein type: Lyase; Amino Acid Metabolism - tryptophan; Nuclear receptor co-regulator; Amino Acid Metabolism - phenylalanine; Amino Acid Metabolism - histidine; Amino Acid Metabolism - tyrosine; EC 4.1.1.28. Chromosomal Location of Human Ortholog: 7p12.2. Cellular Component: synaptic vesicle; cell soma; axon; cytosol. Molecular Function: protein domain specific binding; amino acid binding; aromatic-L-amino-acid decarboxylase activity; protein binding; enzyme binding; pyridoxal phosphate binding. Biological Process: amino acid metabolic process; circadian rhythm; catecholamine biosynthetic process; indolalkylamine biosynthetic process; multicellular organismal aging; isoquinoline alkaloid metabolic process; dopamine biosynthetic process; serotonin biosynthetic process; synaptic vesicle amine transport; phytoalexin metabolic process; response to pyrethroid. Disease: Aromatic L-amino Acid Decarboxylase Deficiency

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage