Rat Annexin A1 ELISA Kit | MyBioSource MBS730221 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Annexin A1 ELISA Kit

catalog :

MBS730221

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS730221

products type :

ELISA Kit

products full name :

Rat Annexin A1 ELISA Kit

products short name :

Annexin A1

other names :

angiopoietin-2 isoform c; Angiopoietin-2; angiopoietin-2; ANG-2; Tie2-ligand; angiopoietin-2B; angiopoietin-2a; angiopoietin 2

products gene name :

ANXA1

other gene names :

ANGPT2; ANGPT2; ANG2; AGPT2; ANG-2

uniprot entry name :

ANGP2_HUMAN

reactivity :

Rat

specificity :

This assay has high sensitivity and excellent specificity for detection of ANXA1. No significant cross-reactivity or interference between ANXA1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ANXA1 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

other info2 :

Intended Uses: This ANXA1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat ANXA1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Cell Biology

products description :

Principle of the assay: ANXA1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ANXA1 antibody and an ANXA1-HRP conjugate. The assay sample and buffer are incubated together with ANXA1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ANXA1 concentration since ANXA1 from samples and ANXA1-HRP conjugate compete for the anti-ANXA1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ANXA1 from the sample, fewer sites are left to bind ANXA1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ANXA1 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

169646754

ncbi acc num :

NP_001112360.1

ncbi gb acc num :

NM_001118888.1

ncbi mol weight :

56,848 Da

ncbi pathways :

Angiopoietin Receptor Tie2-mediated Signaling Pathway (137917); Cell Surface Interactions At The Vascular Wall Pathway (106062); HIF-1 Signaling Pathway (695200); Hemostasis Pathway (106028); PI3K-Akt Signaling Pathway (692234); PI3K-Akt Signaling Pathway (692979); Rap1 Signaling Pathway (868086); Rap1 Signaling Pathway (878042); Ras Signaling Pathway (868085); Tie2 Signaling Pathway (106063)

ncbi summary :

The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

ANGPT2: Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal. Interacts with TEK/TIE2, competing for the same binding site as ANGPT1. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 8p23.1. Cellular Component: extracellular space; cell projection; plasma membrane; extracellular region; nucleus. Molecular Function: protein binding; metal ion binding; receptor tyrosine kinase binding; receptor binding. Biological Process: Tie receptor signaling pathway; organ regeneration; maternal process involved in pregnancy; signal transduction; negative regulation of blood vessel endothelial cell migration; response to organic cyclic substance; germ cell development; negative regulation of positive chemotaxis; negative regulation of angiogenesis; positive regulation of angiogenesis; response to radiation; response to mechanical stimulus; response to glucose stimulus; response to hypoxia; response to activity; angiogenesis; blood coagulation; leukocyte migration

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage