Human Growth Differentiation Factor 11 ELISA Kit | MyBioSource MBS730218 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Growth Differentiation Factor 11 ELISA Kit

catalog :

MBS730218

quantity :

48-Strip-Wells-(Comp

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS730218

products type :

ELISA Kit

products full name :

Human Growth Differentiation Factor 11 ELISA Kit

products short name :

Growth Differentiation Factor 11

other names :

growth/differentiation factor 11; Growth/differentiation factor 11; growth/differentiation factor 11; GDF-11; bone morphogenetic protein 11; growth differentiation factor 11; Bone morphogenetic protein 11; BMP-11

products gene name :

GDF11

other gene names :

GDF11; GDF11; BMP11; BMP-11; BMP11; GDF-11; BMP-11

uniprot entry name :

GDF11_HUMAN

reactivity :

Human

specificity :

This assay has high sensitivity and excellent specificity for detection of GDF-11. No significant cross-reactivity or interference between GDF-11 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GDF-11 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 0.1 ng/mL.

other info2 :

Intended Uses: This GDF-11 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human GDF-11. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Cancer

products description :

Principle of the assay: GDF-11 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for GDF-11. Standards or samples are then added to the microtiter plate wells and GDF-11 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of GDF-11 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for GDF-11 are added to each well to "sandwich" the GDF-11 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain GDF-11 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GDF-11 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

5031613

ncbi acc num :

NP_005802.1

ncbi gb acc num :

NM_005811.3

ncbi mol weight :

45,091 Da

ncbi summary :

The protein encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in mice and Xenopus suggest that this protein is involved in mesodermal formation and neurogenesis during embryonic development. [provided by RefSeq, Jul 2008]

uniprot summary :

GDF11: Secreted signal that acts globally to specify positional identity along the anterior/posterior axis during development. Play critical roles in patterning both mesodermal and neural tissues and in establishing the skeletal pattern. Belongs to the TGF-beta family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 12q13.2. Cellular Component: extracellular space. Molecular Function: growth factor activity; cytokine activity; transforming growth factor beta receptor binding. Biological Process: nervous system development; cell maturation; palate development; regulation of apoptosis; negative regulation of cell proliferation; negative regulation of cell differentiation; camera-type eye morphogenesis; spinal cord anterior/posterior patterning; ureteric bud development; regulation of MAPKKK cascade; pancreas development; mesoderm development; cell development; skeletal development; metanephros development; growth

size1 :

48-Strip-Wells-(Competitive)

price1 :

470 USD

size2 :

48-Strip-Wells-(Sandwich)

price2 :

470

size3 :

96-Strip-Wells-(Competitive)

price3 :

675

size4 :

96-Strip-Wells-(Sandwich)

price4 :

675

more info or order :

MyBioSource product webpage