ELISA/assay

Mouse Aldolase A, Fructose Bisphosphate ELISA Kit

MBS730115

48-Strip-Wells

470 USD

ELISA Kit

Mouse Aldolase A, Fructose Bisphosphate ELISA Kit

Aldolase A, Fructose Bisphosphate

ALDOA, partial; Fructose-bisphosphate aldolase A; fructose-bisphosphate aldolase A; muscle-type aldolase; lung cancer antigen NY-LU-1; fructose-1,6-bisphosphate triosephosphate-lyase; epididymis secretory sperm binding protein Li 87p; aldolase A, fructose-bisphosphate; Lung cancer antigen NY-LU-1; Muscle-type aldolase

ALDOA

ALDOA; ALDOA; ALDA; GSD12; HEL-S-87p; ALDA

ALDOA_HUMAN

Mouse

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture superna tants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate . INTENDED USE This ALDOA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ALDOA. This ELISA kit for research use only, not for therapeutic or diagnostic applications! . PRINCIPLE OF THE ASSAY ALDOA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ALDOA antibody and an ALDOA-HRP conjugate. The assay sample and buffer are incubated together with ALDOA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ALDOA concentration since ALDOA from samples and ALDOA-HRP conjugate compete for the anti-ALDOA antibody binding site. Since the number of sites is limited, as more sites are occupied by ALDOA from the sample, fewer sites are left to bind ALDOA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ALDOA concentration in each sample is interpolated from this standard curve.

49456715

CAG46678.1

P04075

45,261 Da

Biosynthesis Of Amino Acids Pathway (790012); Biosynthesis Of Amino Acids Pathway (795174); Carbon Metabolism Pathway (814926); Carbon Metabolism Pathway (817567); Disease Pathway (530764); Fructose And Mannose Metabolism Pathway (82930); Fructose And Mannose Metabolism Pathway (291); Gluconeogenesis Pathway (106204); Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway (413342); Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway (468196)

The protein encoded by this gene, Aldolase A (fructose-bisphosphate aldolase), is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing and alternative promoter usage results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 3 and 10. [provided by RefSeq, Aug 2011]

ALDOA: a glycolytic enzyme that catalyzes D-fructose 1,6-bisphosphate - glycerone phosphate + D-glyceraldehyde 3-phosphate. Three forms of aldolase are found in vertebrates - aldolase A in muscle, aldolase B in liver and aldolase C in brain. Protein type: Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Lyase; Mitochondrial; EC 4.1.2.13; Carbohydrate Metabolism - pentose phosphate pathway. Chromosomal Location of Human Ortholog: 16p11.2. Cellular Component: I band; extracellular space; membrane; extracellular region; M band; cytosol; nucleus; actin cytoskeleton. Molecular Function: tubulin binding; identical protein binding; protein binding; cytoskeletal protein binding; fructose-bisphosphate aldolase activity; actin binding. Biological Process: platelet activation; striated muscle contraction; glycolysis; actin filament organization; glucose metabolic process; pathogenesis; protein homotetramerization; gluconeogenesis; muscle maintenance; regulation of cell shape; fructose 1,6-bisphosphate metabolic process; platelet degranulation; ATP biosynthetic process; carbohydrate metabolic process; blood coagulation; fructose metabolic process. Disease: Glycogen Storage Disease Xii

48-Strip-Wells

470 USD

96-Strip-Wells

675