ELISA/assay

Human Chemokine C C Motif Ligand 16 ELISA Kit

MBS730047

48-Strip-Wells

470 USD

ELISA Kit

Human Chemokine C C Motif Ligand 16 ELISA Kit

Chemokine C C Motif Ligand 16

C-C motif chemokine 16; C-C motif chemokine 16; C-C motif chemokine 16; monotactin-1; chemokine LEC; chemokine CC-4; new CC chemokine 4; liver CC chemokine-1; IL-10-inducible chemokine; liver-expressed chemokine; small-inducible cytokine A16; lymphocyte and monocyte chemoattractant; small inducible cytokine subfamily A (Cys-Cys), member 16; chemokine (C-C motif) ligand 16; Chemokine CC-4; HCC-4; Chemokine LEC; IL-10-inducible chemokine; LCC-1; Liver-expressed chemokine; Lymphocyte and monocyte chemoattractant; LMC; Monotactin-1; MTN-1; NCC-4; Small-inducible cytokine A16

CCL16

CCL16; CCL16; LEC; LMC; NCC4; CKb12; HCC-4; LCC-1; Mtn-1; NCC-4; SCYL4; ILINCK; SCYA16; ILINCK; NCC4; SCYA16; HCC-4; LMC; MTN-1

CCL16_HUMAN

Human

Store all reagents at 2-8 degree C.

Assay Type: Sandwich

Immunology

Intended Uses: This CCL16 ELISA kit is intended Laboratory for research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofCCL16 in the sample, thisCCL16 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusCCL16 concentration. The concentration ofCCL16 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Principle of the Assay: This CCL16 enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific forCCL16. Standards or samples are then added to the microtiter plate wells andCCL16 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount ofCCL16 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific forCCL16 are added to each well to "sandwich" theCCL16 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that containCCL16 and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.

4759074

NP_004581.1

NM_004590.2

13,600 Da

Chemokine Receptors Bind Chemokines Pathway (106359); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); G Alpha (i) Signalling Events Pathway (119550); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Peptide Ligand-binding Receptors Pathway (106358)

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for lymphocytes and monocytes but not for neutrophils. This cytokine also shows a potent myelosuppressive activity and suppresses proliferation of myeloid progenitor cells. The expression of this gene is upregulated by IL-10. [provided by RefSeq, Jul 2008]

CCL16: Shows chemotactic activity for lymphocytes and monocytes but not neutrophils. Also shows potent myelosuppressive activity, suppresses proliferation of myeloid progenitor cells. Recombinant SCYA16 shows chemotactic activity for monocytes and THP-1 monocytes, but not for resting lymphocytes and neutrophils. Induces a calcium flux in THP-1 cells that were desensitized by prior expression to RANTES. Belongs to the intercrine beta (chemokine CC) family. Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Chemokine; Secreted. Chromosomal Location of Human Ortholog: 17q12. Cellular Component: extracellular space; extracellular region. Molecular Function: chemokine activity; chemoattractant activity. Biological Process: positive chemotaxis; cell-cell signaling; immune response; cell communication; inflammatory response; chemotaxis

48-Strip-Wells

470 USD

96-Strip-Wells

675