ELISA/assay

Rat Anti-nuclear Antibody ELISA Kit

MBS730007

48-Strip-Wells

470 USD

ELISA Kit

Rat Anti-nuclear Antibody ELISA Kit

Anti-nuclear

Rat Anti-nuclear Antibody ELISA Kit; Anti-nuclear; Anti-nuclear Antibody (Rat)

ANA; Protein BTG3; protein BTG3; protein Tob5; OTTHUMP00000083229; OTTHUMP00000083230; B-cell translocation gene 3; abundant in neuroepithelium area protein; BTG family, member 3; Abundant in neuroepithelium area protein; BTG family member 3; Protein Tob5

ANA

BTG3; BTG3; ANA; TOB5; TOFA; TOB55; MGC8928; ANA; TOB5

BTG3_HUMAN

Rat

This assay has high sensitivity and excellent specificity for detection of ANAb. No significant cross-reactivity or interference between ANAb and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ANAb and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This ANAb ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat ANAb. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Rat ELISA Kit

Principle of the assay: ANAb ELISA kit applies the competitive enzyme immunoassay technique utilizing Nuclear antigen and an ANAb-HRP conjugate. The assay sample and buffer are incubated together with ANAb-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ANAb concentration since ANAb from samples and ANAb-HRP conjugate compete for the Nuclear antigen binding site. Since the number of sites is limited, as more sites are occupied by ANAb from the sample, fewer sites are left to bind ANAb-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ANAb concentration in each sample is interpolated from this standard curve.

3738222

Q14201

29,116 Da

RNA Degradation Pathway (117291); RNA Degradation Pathway (116127)

The protein encoded by this gene is a member of the BTG/Tob family. This family has structurally related proteins that appear to have antiproliferative properties. This encoded protein might play a role in neurogenesis in the central nervous system. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

BTG3: Overexpression impairs serum-induced cell cycle progression from the G0/G1 to S phase. Belongs to the BTG family. 2 isoforms of the human protein are produced by alternative splicing. Chromosomal Location of Human Ortholog: 21q21.1. Cellular Component: cytoplasm. Molecular Function: protein binding. Biological Process: negative regulation of cell proliferation; negative regulation of mitotic cell cycle

48-Strip-Wells

470 USD

96-Strip-Wells

675