ELISA/assay

Mouse Steroid 5 Alpha Reductase ELISA Kit

MBS729961

48-Strip-Wells

470 USD

ELISA Kit

Mouse Steroid 5 Alpha Reductase ELISA Kit

Steroid 5 Alpha Reductase

steroid 5-alpha reductase; Steroid 5-alpha reductase; very-long-chain enoyl-CoA reductase-like; steroid 5-alpha reductase; Steroid 5-alpha reductase

SRD5a

LOC101508101; sr5a; sr5a

Q8GTD7_CICAR

Mouse

This assay has high sensitivity and excellent specificity for detection of SRD5a. No significant cross-reactivity or interference between SRD5a and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SRD5a and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This SRD5a ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse SRD5a. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the assay: SRD5a ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SRD5a antibody and an SRD5a-HRP conjugate. The assay sample and buffer are incubated together with SRD5a-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SRD5a concentration since SRD5a from samples and SRD5a-HRP conjugate compete for the anti-SRD5a antibody binding site. Since the number of sites is limited, as more sites are occupied by SRD5a from the sample, fewer sites are left to bind SRD5a-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SRD5a concentration in each sample is interpolated from this standard curve.

25004880

CAD56504.1

Q8GTD7

35,989 Da

Biosynthesis Of Secondary Metabolites Pathway (873102); Biosynthesis Of Unsaturated Fatty Acids Pathway (873100); Biosynthesis Of Unsaturated Fatty Acids Pathway (429); Fatty Acid Biosynthesis, Elongation, Endoplasmic Reticulum Pathway (885111); Fatty Acid Biosynthesis, Elongation, Endoplasmic Reticulum Pathway (468402); Fatty Acid Elongation Pathway (873022); Fatty Acid Elongation Pathway (295); Fatty Acid Metabolism Pathway (873137); Fatty Acid Metabolism Pathway (878045); Metabolic Pathways (873106)

48-Strip-Wells

470 USD

96-Strip-Wells

675