ELISA/assay

Rat Mucin-5 subtype AC ELISA Kit

MBS729889

48-Strip-Wells-(Competitive)

440 USD

ELISA Kit

Rat Mucin-5 subtype AC ELISA Kit

[Mucin-5 subtype AC]

[MUC5AC protein, partial; MUC5AC protein; MUC5AC protein]

[MUC5AC]

[MUC5AC]

Q96DU6_HUMAN

Rat

This assay has high sensitivity and excellent specificity for detection of MUC5AC. No significant cross-reactivity or interference between MUC5AC and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MUC5AC and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate. Assay Type: Quantitative Competitive. Sensitivity: 1.0 pg/mL.

Signal Transduction

Intended Uses: This MUC5AC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine MUC5AC. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: MUC5AC ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-MUC5AC antibody and an MUC5AC-HRP conjugate. The assay sample and buffer are incubated together with MUC5AC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MUC5AC concentration since MUC5AC from samples and MUC5AC-HRP conjugate compete for the anti-MUC5AC antibody binding site. Since the number of sites is limited, as more sites are occupied by MUC5AC from the sample, fewer sites are left to bind MUC5AC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MUC5AC concentration in each sample is interpolated from this standard curve.

15026993

CAC44892.1

6,501 Da

48-Strip-Wells-(Competitive)

440 USD

48-Strip-Wells-(Sandwich)

440

96-Strip-Wells-(Competitive)

640

96-Strip-Wells-(Sandwich)

640

5x96-Strip-Wells-(Competitive)

2895

5x96-Strip-Wells-(Sandwich)

2895

10x96-Strip-Wells-(Competitive)

5415

10x96-Strip-Wells-(Sandwich)

5415