ELISA/assay

Rat T lymphocyte subpopulation CD3/4/8 ELISA Kit

MBS729871

96 Strip Wells

675 USD

ELISA Kit

Rat T lymphocyte subpopulation CD3/4/8 ELISA Kit

T lymphocyte subpopulation CD3/4/8

TLS; RNA-binding protein FUS; RNA-binding protein FUS; oncogene FUS; oncogene TLS; fus-like protein; fused in sarcoma; 75 kDa DNA-pairing protein; fusion gene in myxoid liposarcoma; translocated in liposarcoma protein; heterogeneous nuclear ribonucleoprotein P2; FUS RNA binding protein; 75 kDa DNA-pairing protein; Oncogene FUS; Oncogene TLS; POMp75; Translocated in liposarcoma protein

TLS

FUS; FUS; TLS; ALS6; ETM4; FUS1; POMP75; HNRNPP2; TLS

FUS_HUMAN

Rat

Store all reagents at 2-8 degree C.

Sensitivity: The sensitivity in this assay is 0.1 ng/mL.

Immunology

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate . INTENDED USE This TLS ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat TLS. This ELISA kit for research use only, not for therapeutic or diagnostic applications! . PRINCIPLE OF THE ASSAY TLS ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TLS antibody and an TLS-HRP conjugate. The assay sample and buffer are incubated together with TLS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TLS concentration since TLS from samples and TLS-HRP conjugate compete for the anti-TLS antibody binding site. Since the number of sites is limited, as more sites are occupied by TLS from the sample, fewer sites are left to bind TLS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TLS concentration in each sample is interpolated from this standard curve.

386157

AAB27102.1

53,355 Da

Gene Expression Pathway 105937!!Processing Of Capped Intron-Containing Pre-mRNA Pathway 160950!!Transcriptional Misregulation In Cancer Pathway 523016!!Transcriptional Misregulation In Cancer Pathway 522987!!MRNA Splicing Pathway 105951!!MRNA Splicing - Major Pathway 105952!!MRNA Processing Pathway 198843

This gene encodes a multifunctional protein component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complex. The hnRNP complex is involved in pre-mRNA splicing and the export of fully processed mRNA to the cytoplasm. This protein belongs to the FET family of RNA-binding proteins which have been implicated in cellular processes that include regulation of gene expression, maintenance of genomic integrity and mRNA/microRNA processing. Alternative splicing results in multiple transcript variants. Defects in this gene result in amyotrophic lateral sclerosis type 6. [provided by RefSeq, Sep 2009]

96 Strip Wells

675 USD