ELISA/assay

Rat Matrix Metalloproteinase 11 ELISA Kit

MBS729806

48-Strip-Wells-(Comp

470 USD

ELISA Kit

Rat Matrix Metalloproteinase 11 ELISA Kit

Matrix Metalloproteinase 11

matrix metalloproteinase-11, partial; Matrix metalloproteinase-11; stromelysin-3; matrix metalloproteinase 11; Matrix metalloproteinase-11

MMP-11

MMP11; MMP-11

D6R1X0_CANFA

Rat

Store all reagents at 2-8 degree C.

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 0.1 ng/mL.

Cancer

Intended Uses: This MMP-11 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat MMP-11. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: MMP-11 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for MMP-11. Standards or samples are then added to the microtiter plate wells and MMP-11 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of MMP-11 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for MMP-11 are added to each well to "sandwich" the MMP-11 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain MMP-11 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MMP-11 concentration in each sample is interpolated from this standard curve.

295983604

ADG63456.1

11,465 Da

Activation Of Matrix Metalloproteinases Pathway (1016248); Collagen Degradation Pathway (1016249); Degradation Of The Extracellular Matrix Pathway (1016247); Extracellular Matrix Organization Pathway (1016234); Matrix Metalloproteinases Pathway (198594)

48-Strip-Wells-(Competitive)

470 USD

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675