ELISA/assay

Mouse Annexin V ELISA Kit

MBS729773

48-Strip-Wells

470 USD

ELISA Kit

Mouse Annexin V ELISA Kit

Annexin V

annexin A5; annexin A5; annexin 5; annexin A5b

ANX-V

anxa5b; anx; anx5; ANX V; anxa5; cb989; wu:fa98f06; wu:fj10f10

Mouse

This assay has high sensitivity and excellent specificity for detection of ANXA-5. No significant cross-reactivity or interference between ANXA-5 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ANXA-5 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Quantitative Sandwich. Sensitivity: 1.0pg/mL.

Cell Biology

Intended Uses: This ANXA-5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ANXA-5. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: ANXA-5 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ANXA-5. Standards or samples are then added to the microtiter plate wells and ANXA-5 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ANXA-5 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ANXA-5 are added to each well to “sandwich” the ANXA-5 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ANXA-5 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ANXA-5 concentration in each sample is interpolated from this standard curve.

40254661

NP_861422.2

NM_181757.2

48-Strip-Wells

470 USD

96-Strip-Wells

675