ELISA/assay

Mouse Placenta Growth Factor ELISA Kit

MBS729740

48-Strip-Wells

470 USD

ELISA Kit

Mouse Placenta Growth Factor ELISA Kit

Placenta Growth Factor

placenta growth factor isoform 1; Placenta growth factor; placenta growth factor; placental growth factor

PLGF

Pgf; Pgf; PIGF; Plgf; AI854365; Plgf; PlGF

PLGF_MOUSE

Mouse

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell cult re supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Intended Uses: This PLGF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse PLGF. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: PLGF ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PLGF antibody and an PLGF-HRP conjugate. The assay sample and buffer are incubated together with PLGF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PLGF concentration since PLGF from samples and PLGF-HRP conjugate compete for the anti-PLGF antibody binding site. Since the number of sites is limited, as more sites are occupied by PLGF from the sample, fewer sites are left to bind PLGF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PLGF concentration in each sample is interpolated from this standard curve.

6679289

NP_032853.1

NM_008827.3

17,876 Da

Focal Adhesion Pathway (198353); Focal Adhesion Pathway (83264); Focal Adhesion Pathway (478); PI3K-Akt Signaling Pathway (692242); PI3K-Akt Signaling Pathway (692979); Pathways In Cancer (83298); Rap1 Signaling Pathway (869018); Rap1 Signaling Pathway (878042); Ras Signaling Pathway (869019); Signal Transduction Pathway (970540)

PGF: Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. Also promotes cell tumor growth. Belongs to the PDGF/VEGF growth factor family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted. Cellular Component: extracellular space; membrane; extracellular region. Molecular Function: protein homodimerization activity; growth factor activity; protein heterodimerization activity. Biological Process: positive regulation of angiogenesis; ureteric bud branching; multicellular organismal development; positive regulation of cell division; positive regulation of cell proliferation; positive regulation of endothelial cell proliferation; sprouting angiogenesis; angiogenesis; cell differentiation

48-Strip-Wells

470 USD

96-Strip-Wells

675