ELISA/assay

Rat c-Fos ELISA Kit

MBS729725

48-Strip-Wells

470 USD

ELISA Kit

Rat c-Fos ELISA Kit

c-Fos

c-fos; Proto-oncogene c-Fos; proto-oncogene c-Fos; activator protein 1; cellular oncogene c-fos; G0/G1 switch regulatory protein 7; FBJ murine osteosarcoma viral (v-fos) oncogene homolog (oncogene FOS); FBJ murine osteosarcoma viral oncogene homolog; Cellular oncogene fos; G0/G1 switch regulatory protein 7

c-Fos

FOS; FOS; p55; AP-1; C-FOS; G0S7

FOS_HUMAN

Rat

This assay has high sensitivity and excellent specificity for detection of c-Fos. No significant cross-reactivity or interference between c-Fos and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between c-Fos and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This c-Fos ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat c-Fos. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Neurobiology

Principle of the Assay: c-Fos ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-c-Fos antibody and an c-Fos-HRP conjugate. The assay sample and buffer are incubated together with c-Fos-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the c-Fos concentration since c-Fos from samples and c-Fos-HRP conjugate compete for the anti-c-Fos antibody binding site. Since the number of sites is limited, as more sites are occupied by c-Fos from the sample, fewer sites are left to bind c-Fos-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The c-Fos concentration in each sample is interpolated from this standard curve.

29904

CAA24756.1

P01100

36,301 Da

ATF-2 Transcription Factor Network Pathway (138006); Activated TLR4 Signalling Pathway (106400); Activation Of The AP-1 Family Of Transcription Factors Pathway (160139); Amphetamine Addiction Pathway (547607); Amphetamine Addiction Pathway (550546); Apoptosis Modulation And Signaling Pathway (198822); B Cell Receptor Signaling Pathway (83081); B Cell Receptor Signaling Pathway (492); BCR Signaling Pathway (138058); BDNF Signaling Pathway (712093)

The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. [provided by RefSeq, Jul 2008]

Fos: a proto-oncogenic transcription factor of the bZIP family. Dimerizes with proteins of the JUN family, thereby forming the transcription factor complex AP-1. FOS proteins function as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of FOS has also been associated with apoptotic cell death. Expression increases upon a variety of stimuli, including growth factors, cytokines, neurotransmitters, polypeptide hormones, stress and cell injury. Protein type: Motility/polarity/chemotaxis; Oncoprotein; DNA-binding; Transcription factor. Chromosomal Location of Human Ortholog: 14q24.3. Cellular Component: nucleoplasm; transcription factor complex; neuron projection; membrane; endoplasmic reticulum; cytosol; nucleus. Molecular Function: protein binding; double-stranded DNA binding; transcription factor binding; transcription factor activity. Biological Process: transcription from RNA polymerase II promoter; response to gravity; response to cAMP; positive regulation of osteoclast differentiation; positive regulation of transcription, DNA-dependent; response to toxin; stress-activated MAPK cascade; response to lipopolysaccharide; toll-like receptor 3 signaling pathway; female pregnancy; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; regulation of transcription factor activity; conditioned taste aversion; transforming growth factor beta receptor signaling pathway; DNA methylation; inflammatory response; toll-like receptor 4 signaling pathway; response to corticosterone stimulus; aging; response to drug; response to light stimulus; nervous system development; MyD88-independent toll-like receptor signaling pathway; sleep; toll-like receptor 2 signaling pathway; cellular response to hormone stimulus; regulation of transcription from RNA polymerase II promoter; MyD88-dependent toll-like receptor signaling pathway; response to mechanical stimulus; response to cytokine stimulus; cellular response to extracellular stimulus; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; response to cold; toll-like receptor 9 signaling pathway; response to progesterone stimulus

48-Strip-Wells

470 USD

96-Strip-Wells

675