catalog number :
MBS729656
products type :
ELISA Kit
products full name :
Human Hemoglobin A ELISA Kit
products short name :
Hemoglobin A
other names :
hemoglobin A; Hemoglobin A; Hemoglobin A
uniprot entry name :
I2G907_9HEMI
specificity :
This assay has high sensitivity and excellent specificity for detection of HBA. No significant cross-reactivity or interference between HBA and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HBA and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ug/mL.
other info2 :
Intended Uses: This HBA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human HBA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products description :
Principle of the assay: HBA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HBA antibody and an HBA-HRP conjugate. The assay sample and buffer are incubated together with HBA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HBA concentration since HBA from samples and HBA-HRP conjugate compete for the anti-HBA antibody binding site. Since the number of sites is limited, as more sites are occupied by HBA from the sample, fewer sites are left to bind HBA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HBA concentration in each sample is interpolated from this standard curve.
ncbi acc num :
CCG97778.1
ncbi mol weight :
17,668 Da