ELISA/assay

Human Glucokinase ELISA Kit

MBS729636

48-Strip-Wells-(Comp

470 USD

ELISA Kit

Human Glucokinase ELISA Kit

Glucokinase

glucokinase isoform 2; Glucokinase; glucokinase; HK4; HK IV; hexokinase-4; hexokinase-D; hexokinase type IV; glucokinase variant 3; glucokinase; Hexokinase type IV; HK IV; Hexokinase-4; HK4; Hexokinase-D

GCK

Gck; Gck; GLUKA; RNGK2; HK IV; HK4

HXK4_RAT

Human

This assay has high sensitivity and excellent specificity for detection of GCK. No significant cross-reactivity or interference between GCK and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GCK and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 0.1 ng/mL.

Intended Uses: This GCK ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human GCK. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the assay: GCK ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for GCK. Standards or samples are then added to the microtiter plate wells and GCK if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of GCK present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for GCK are added to each well to "sandwich" the GCK immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain GCK and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GCK concentration in each sample is interpolated from this standard curve.

7110599

NP_036697.1

NM_012565.2

51,919 Da

Amino Sugar And Nucleotide Sugar Metabolism Pathway (83371); Amino Sugar And Nucleotide Sugar Metabolism Pathway (350); Butirosin And Neomycin Biosynthesis Pathway (145805); Butirosin And Neomycin Biosynthesis Pathway (145786); Carbon Metabolism Pathway (816129); Carbon Metabolism Pathway (817567); Developmental Biology Pathway (1009870); Disease Pathway (1010484); Galactose Metabolism Pathway (83324); Galactose Metabolism Pathway (292)

catalyzes the conversion of ATP and D-glucose to ADP and D-glucose 6-phosphate; may mediate glucosensing in glucose-inhibited and glucose-excited neurons [RGD, Feb 2006]

48-Strip-Wells-(Competitive)

470 USD

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675