catalog number :
MBS729586
products type :
ELISA Kit
products full name :
Rabbit alpha Melanocyte Stimulating Hormone ELISA Kit
products short name :
alpha Melanocyte Stimulating Hormone
products name syn :
Rabbit a Melanocyte Stimulating Hormone ELISA Kit
other names :
alpha-melanocyte-stimulating hormone, partial; Alpha-melanocyte-stimulating hormone; Alpha-melanocyte-stimulating hormone
products gene name :
alpha-MSH
products gene name syn :
a-MSH
uniprot entry name :
D2JZU1_CAPHI
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.
other info2 :
Intended Uses: This alpha-MSH ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit alpha-MSH. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products categories :
Signal Transduction
products description :
Principle of the Assay: alpha-MSH ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-alpha-MSH antibody and an alpha-MSH-HRP conjugate. The assay sample and buffer are incubated together with alpha-MSH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the alpha-MSH concentration since alpha-MSH from samples and alpha-MSH-HRP conjugate compete for the anti-alpha-MSH antibody binding site. Since the number of sites is limited, as more sites are occupied by alpha-MSH from the sample, fewer sites are left to bind alpha-MSH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The alpha-MSH concentration in each sample is interpolated from this standard curve.
ncbi acc num :
ACZ56127.1
ncbi mol weight :
5,602 Da