ELISA/assay

Human S100 calcium binding protein beta ELISA Kit

MBS729573

48-Strip-Wells-(Comp

470 USD

ELISA Kit

Human S100 calcium binding protein beta ELISA Kit

S100 calcium binding protein beta

Human S100 calcium binding protein b ELISA Kit

S100 protein beta subunit; Protein S100-B; protein S100-B; S-100 protein subunit beta; S-100 calcium-binding protein, beta chain; S100 calcium-binding protein, beta (neural); S100 calcium binding protein B; S-100 protein beta chain; S-100 protein subunit beta; S100 calcium-binding protein B

S100beta

S100b

S100B; S100B; NEF; S100; S100-B; S100beta

S100B_HUMAN

Human

Store all reagents at 2-8 degree C.

Assay Type: Competitive or Sandwich. Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Sensitivity: 0.1 ng/mL.

Intended Uses: This S100B ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human S100B. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Signal Transduction

Principle of the Assay: S100B ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for S100B. Standards or samples are then added to the microtiter plate wells and S100B if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of S100B present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for S100B are added to each well to "sandwich" the S100B immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain S100B and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The S100B concentration in each sample is interpolated from this standard curve.

337730

AAA60367.1

10,713 Da

Activated TLR4 Signalling Pathway (106400); Advanced Glycosylation Endproduct Receptor Signaling Pathway (187092); Cytosolic Sensors Of Pathogen-associated DNA Pathway (576255); DEx/H-box Helicases Activate Type I IFN And Inflammatory Cytokines Production Pathway (833822); Immune System Pathway (106386); Innate Immune System Pathway (106387); MyD88 Cascade Initiated On Plasma Membrane Pathway (205107); MyD88 Dependent Cascade Initiated On Endosome Pathway (187081); MyD88-independent Cascade Pathway (106401); MyD88:Mal Cascade Initiated On Plasma Membrane Pathway (106390)

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in Neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes. [provided by RefSeq, Jul 2008]

S100B: Weakly binds calcium but binds zinc very tightly- distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain. The S100B dimer binds two molecules of STK38. Interacts with AGER. The S100B dimer interacts with two molecules of CAPZA1. Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues. Belongs to the S-101 family. Protein type: Calcium-binding. Chromosomal Location of Human Ortholog: 21q22.3. Cellular Component: ruffle; extracellular space; cell soma; intracellular membrane-bound organelle; perinuclear region of cytoplasm; cytoplasm; extracellular region; nucleus. Molecular Function: identical protein binding; protein binding; RAGE receptor binding; protein homodimerization activity; zinc ion binding; calcium ion binding; calcium-dependent protein binding; tau protein binding. Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; central nervous system development; positive regulation of apoptosis; response to glucocorticoid stimulus; memory; regulation of cell shape; cell proliferation; learning and/or memory; astrocyte differentiation; axonogenesis; positive regulation of cell proliferation; response to methylmercury; innate immune response; regulation of neuronal synaptic plasticity

48-Strip-Wells-(Competitive)

470 USD

48-Strip-Wells-(Sandwich)

470

96-Strip-Wells-(Competitive)

675

96-Strip-Wells-(Sandwich)

675