catalog number :
MBS729453
products type :
ELISA Kit
products full name :
Human Glycogen ELISA Kit
products short name :
[Glycogen]
other names :
[Glycogen, partial; Glycogen; Glycogen]
products gene name :
[Glycogen]
uniprot entry name :
S4PV39_9NEOP
specificity :
This assay has high sensitivity and excellent specificity for detection of GLUT4. No significant cross-reactivity or interference between GLUT4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GLUT4 and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate. Assay Type: Quantitative Competitive. Sensitivity: 0.1 ng/mL.
products description :
Intended Uses: This GLUT4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of MouseGLUT4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: GLUT4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-GLUT4 antibody and an GLUT4-HRP conjugate. The assay sample and buffer are incubated together with GLUT4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GLUT4 concentration since GLUT4 from samples and GLUT4-HRP conjugate compete for the anti-GLUT4 antibody binding site. Since the number of sites is limited, as more sites are occupied by GLUT4 from the sample, fewer sites are left to bind GLUT4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GLUT4 concentration in each sample is interpolated from this standard curve.
ncbi acc num :
JAA83352.1
ncbi mol weight :
15,415 Da
size4 :
10x96-Strip-Wells