catalog number :
MBS729421
products type :
ELISA Kit
products full name :
Mouse Anti-Ribosomal P Protein Antibody ELISA Kit
products short name :
Anti-Ribosomal P Protein Antibody
other names :
ARPa, partial; ARPa; ARPa
products gene name :
ARPA
uniprot entry name :
C4NFR8_9CARY
specificity :
This assay has high sensitivity and excellent specificity for detection of ARPAb. No significant cross-reactivity or interference between ARPAb and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ARPAb and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ng/mL.
products description :
Intended Uses: This ARPAb ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ARPAb. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay ARPAb ELISA kit applies the competitive enzyme immunoassay technique utilizing Ribosomal P Protein antigen and an ARPAb-HRP conjugate. The assay sample and buffer are incubated together with ARPAb-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ARPAb concentration since ARPAb from samples and ARPAb-HRP conjugate compete for the Ribosomal P Protein antigen binding site. Since the number of sites is limited, as more sites are occupied by ARPAb from the sample, fewer sites are left to bind ARPAb-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ARPAb concentration in each sample is interpolated from this standard curve.
ncbi acc num :
ACQ76775.1
ncbi mol weight :
28,522 Da